Differential Gene Expression of Na, K-ATPase α- and β-Subunits in the Developing Brine Shrimp, Artemia
Na, K-ATPase plays an important role in maintaining Na+ and K+ electrochemical gradients across the plasma membrane . It effects the coupled transports of three Na+ ions out of the cell and two K+ ions into the cell driven by the hydrolysis of one ATP. The ion gradients are essential for osmotic homeostasis and certain physiological processes such as the wave of excitation in nerve and muscle, ion transport in structures such as the kidney and intestine, and ion secretion in glands such as the avian salt gland and salivary glands. The enzyme has been purified from several tissues [2–4]. Numerous studies have shown that the enzyme molecule consists of two subunits: a catalytic α-subunit and a glycosylated β-subunit [2,3,5–7].
KeywordsBrine Shrimp Northern Hybridization Ribonuclease Protection Assay Adenosine Triphosphatase Subunit mRNA
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