Artemia Mitochondrial DNA

  • Roberto Marco
  • Beatriz Batuecas
  • Carmen Moratilla
  • Jesús Diez-Sebastián
  • Ruben Gómez-Rioja
  • Fernando Díaz-Otero
  • Alfonso Valencia
  • Manuel Calleja
  • José Ramón Valverde
  • Rafael Garesse
Part of the NATO ASI Series book series (NSSA, volume 174)


Previous work by members of our group [1,2,3] suggested that in Artemia early development a considerable proportion of the mitochondria were stored in an inactive state in the yolk granules from which they were released during the developmental process, becoming active as power generators in the nauplii. In order to test conclusively this idea, we turned to the mitochondrial DNA as we thought that it could provide the best marker for the presence and quantitation of these organelles, independent of their functional status. Very little was known about this interesting informational molecule in Artemia [4], but in other systems as distant as sea urchin, amphibians and mammals [5,6,7] it is known that it is basically synthesized during oogenesis, the mature oocyte containing all the mitochondria necessary for early embryogenesis.


Mitochondrial Genome tRNA Gene Gene Organization Main Gene Cytochrome Oxidase Subunit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. 1.
    R. Marco, A. Garesse and C. G. Vallejo, Mitochondrial unmasking and yolk platelets metabolization during early development in Artemia salina, in: “The Brine Shrimp Artemia,” Vol. 2, G. Persoone, P. Sorgeloos, D. Roels and E. Jasper, eds., Universa Press, Wetteren (1980).Google Scholar
  2. 2.
    R. Marco, R. Garesse and C. G. Vallejo, Storage of mitochondria in the yolk platelets of Artemia dormant gastrulae, Cell. Mol. Biol. 27:515 (1981).Google Scholar
  3. 3.
    R. Marco, B. Batuecas, M. Calleja, M. Carratalá, M. Cervera, A. Domingo, C. Ferreiro, R. Garesse, C. Urquía and I. Vernós, Understanding the organization of cell metabolism in early embryonic systems. Developmental implications, in: “The Organization of Cell Metabolism,” R. G. Welch and J. G. Clegg, eds., Plenum, New York (1987).Google Scholar
  4. 4.
    H. Schmitt, J. S. Beckmann and U. Z. Littauer, Transcription of supercoiled mitochondrial DNA by bacterial RNA Polymerase, Eur. J. Biochem. 47:227 (1974).CrossRefGoogle Scholar
  5. 5.
    L. Pikó, A. Tyler and J. Vinograd, Amount, location, priming capacity, circularity and other properties of cytoplasmic DNA in sea urchin eggs, Biol. Bull. 132:68 (1967).PubMedCrossRefGoogle Scholar
  6. 6.
    I. B. Dawid, Evolution of Mitochondrial DNA sequences in Xenopus, Develop. Biol. 29:139 (1972).PubMedCrossRefGoogle Scholar
  7. 7.
    G. S. Michaels, W. W. Hauswirth and P. J. Laipis, Mitochondrial DNA copy number in bovine oocytes and somatic cells, Develop. Biol. 94: 246 (1982).PubMedCrossRefGoogle Scholar
  8. 8.
    B. Batuecas, R. Marco, M. Calleja and R. Garesse, Molecular characterization of Artemia mitochondrial DNA: cloning, physical mapping and preliminary gene organization, in: “Artemia Research and its Applications,” Vol. 2, W. Decleir, L. Moens, H. Siegers, E. Jasper and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar
  9. 9.
    D. R. Wolstenholme, J. L. MacFarlaine, R. Okimoto, D. O. Clary and J. A. Wahleithner, Bizarre tRNAs inferred from DNA sequences of mitochondrial genomes of nematode worms, Proc. Natl. Acad. Sci. U.S.A. 84:1324 (1987).PubMedCrossRefGoogle Scholar
  10. 10.
    B. Batuecas, R. Garesse, M. Calleja, J. R. Valverde and R. Marco, Genome Organization of Artemia mitochondrial DNA, Nucleic Acid Res. 16:6515 (1988).PubMedCrossRefGoogle Scholar
  11. 11.
    M. H. L. De Bruijn, Drosophila melanogaster mitochondrial DNA: a novel Organization and genetic code, Nature 304:234 (1983).PubMedCrossRefGoogle Scholar
  12. 12.
    R. Garesse, Drosophila melanogaster mitochondrial DNA: Gene Organization and evolutionary considerations, Genetics 118:649 (1988).PubMedGoogle Scholar
  13. 13.
    D. R. Woltensholme and D. O. Clary, Sequence evolution of Drosophila mitochondrial DNA, Genetics 105:725 (1985).Google Scholar
  14. 14.
    J. M. Goddard and D. R. Woltensholme, Origin and direction of replication in mitochondrial DNA molecules from Drosophila melanogaster, Proc. Natl. Acad. Sci. 75:3886 (1978).PubMedCrossRefGoogle Scholar
  15. 15.
    W. B. Upholt, Estimation of DNA sequence divergence from comparison of restriction endonuclease digests, Nucleic Acid Res. 4:1257 (1977).PubMedCrossRefGoogle Scholar
  16. 16.
    C. Moritz, T. E. Dowling and W. M. Brown, Evolution of animal mitochondria: Relevance of population biology and systematics, Ann. Rev. Ecol. Syst. 18:262 (1987).CrossRefGoogle Scholar
  17. 17.
    R. A. Raff and T. C. Kaufman, “Embryos, Genes and Evolution,” MacMillan New York (1983).Google Scholar
  18. 18.
    F. A. Abreu-Grobois, A review of the genetics of Artemia, in: “Artemia Research and its Applications,” Vol. 2, W. Decler, L. Moens, H. Siegers, E. Jaspers and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar
  19. 19.
    P. Cantatore, M. Roberti, G. Rainadi, C. Saccone and M. N. Gadaleta, Clustering of tRNA genes in Paracentrotus lividus mitochondrial DNA, Current Gen. 13:91 (1988).CrossRefGoogle Scholar
  20. 20.
    H. T. Jacobs, D. J. Elliot, V. B. Math and A. Farquharson, Nucleotide sequence and gene Organization of sea urchin mitochondrial DNA, J. Mol. Biol. 202:185 (1988).PubMedCrossRefGoogle Scholar
  21. 21.
    B. A. Roe, D.-P. Ma, R. K. Wilson and J. F.-H. Wong, The complete nucleotide sequence of the Xenopus laevis mitochondrial genome, J. Biol. Chem. 260:9759 (1985).PubMedGoogle Scholar
  22. 22.
    M. J. Bibb, R. A. Van Etten, C. T. Wright, M. W. Walberg and D. A. Clayton, Sequence and gene organization of mouse mitochondrial genome, Cell 26:167 (1981).PubMedCrossRefGoogle Scholar
  23. 23.
    S. M. Anderson, M. H. L. De Bruijn, A. R. Coulson, J. Drouin, I. C. Eperon, F. Sanger and I. G. Young, The complete sequence of bovine mitochondrial DNA: conserved features of the mammalian mitochondrial genome, J. Mol. Biol. 156:683 (1982).PubMedCrossRefGoogle Scholar
  24. 24.
    S. M. Anderson, A. T. Bankier, B. G. Barreil, M. H. L. De Bruijn, A. R. Coulson, J. Drouin, I. C. Eperon, B. Nierlich, A. Roe, F. Sanger, P. H. Schreier, A. J. H. Smith, R. Staden and I. G. Young, Sequence and organization of human mitochondrial genome, Nature 290:457 (1981).PubMedCrossRefGoogle Scholar
  25. 25.
    A. C. Wilson, V. M. Sarich and L. R. Maxson, The importance of gene rearrangement in evolution, Proc. Nat. Acad. Sci. 71:3028 (1974).PubMedCrossRefGoogle Scholar
  26. 26.
    D. R. Wolstenholme, D. O. Clary, J. L. MacFarlane, S. A. Wahleithener and L. Nilcox, Organization and evolution of invertebrate mitochondrial genomes, in: “Artemia Research and its Applications,” Vol. 2, W. Decleir, L. Moens, H. Siegers, E. Jaspers and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar
  27. 27.
    J. R. Palmer, Comparative organization of chloroplast genomes, Ann. Rev. Genet. 19:325 (1985).PubMedCrossRefGoogle Scholar
  28. 28.
    R. M. Schwartz and M. O. Dayhoff, “Cytochromes Atlas of Protein Sequence and Structure,” Vol. 5, Suppl 3, National Biomedical Foundation, Washington (1978).Google Scholar
  29. 29.
    M. S. Gardiner, “The Biology of Invertebrates,” McGraw-Hill, New York, (1972).Google Scholar
  30. 30.
    K. G. Field, G. J. Olsen, D. J. Lane, S. J. Giovannoni, M. T. Ghiselin, E. C. Raff, N. R. Pace and R. A. Raff, Molecular phylogeny of the animal kingdom, Science 239:748 (1988).PubMedCrossRefGoogle Scholar
  31. 31.
    P. Y. Chou and G. D. Fasman, Empirical predictions of protein conformation, Ann. Rev. Biochem. 47:251 (1978).PubMedCrossRefGoogle Scholar
  32. 32.
    J. Kyte and R. F. Doolittle, A simple method for displaying the hydropathic character of a protein, J. Mol. Biol. 157:105 (1982).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Roberto Marco
    • 1
  • Beatriz Batuecas
    • 1
  • Carmen Moratilla
    • 1
  • Jesús Diez-Sebastián
    • 1
  • Ruben Gómez-Rioja
    • 1
  • Fernando Díaz-Otero
    • 1
  • Alfonso Valencia
    • 1
  • Manuel Calleja
    • 1
  • José Ramón Valverde
    • 1
  • Rafael Garesse
    • 1
  1. 1.Departmento de Bioquímica de la UAM e Instituto de Investigaciones Biomédicas del CSIC Facultad de MedicinaUniversidad AutónomaMadridSpain

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