Artemia Mitochondrial DNA
Previous work by members of our group [1,2,3] suggested that in Artemia early development a considerable proportion of the mitochondria were stored in an inactive state in the yolk granules from which they were released during the developmental process, becoming active as power generators in the nauplii. In order to test conclusively this idea, we turned to the mitochondrial DNA as we thought that it could provide the best marker for the presence and quantitation of these organelles, independent of their functional status. Very little was known about this interesting informational molecule in Artemia , but in other systems as distant as sea urchin, amphibians and mammals [5,6,7] it is known that it is basically synthesized during oogenesis, the mature oocyte containing all the mitochondria necessary for early embryogenesis.
KeywordsMitochondrial Genome tRNA Gene Gene Organization Main Gene Cytochrome Oxidase Subunit
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- 1.R. Marco, A. Garesse and C. G. Vallejo, Mitochondrial unmasking and yolk platelets metabolization during early development in Artemia salina, in: “The Brine Shrimp Artemia,” Vol. 2, G. Persoone, P. Sorgeloos, D. Roels and E. Jasper, eds., Universa Press, Wetteren (1980).Google Scholar
- 2.R. Marco, R. Garesse and C. G. Vallejo, Storage of mitochondria in the yolk platelets of Artemia dormant gastrulae, Cell. Mol. Biol. 27:515 (1981).Google Scholar
- 3.R. Marco, B. Batuecas, M. Calleja, M. Carratalá, M. Cervera, A. Domingo, C. Ferreiro, R. Garesse, C. Urquía and I. Vernós, Understanding the organization of cell metabolism in early embryonic systems. Developmental implications, in: “The Organization of Cell Metabolism,” R. G. Welch and J. G. Clegg, eds., Plenum, New York (1987).Google Scholar
- 8.B. Batuecas, R. Marco, M. Calleja and R. Garesse, Molecular characterization of Artemia mitochondrial DNA: cloning, physical mapping and preliminary gene organization, in: “Artemia Research and its Applications,” Vol. 2, W. Decleir, L. Moens, H. Siegers, E. Jasper and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar
- 13.D. R. Woltensholme and D. O. Clary, Sequence evolution of Drosophila mitochondrial DNA, Genetics 105:725 (1985).Google Scholar
- 17.R. A. Raff and T. C. Kaufman, “Embryos, Genes and Evolution,” MacMillan New York (1983).Google Scholar
- 18.F. A. Abreu-Grobois, A review of the genetics of Artemia, in: “Artemia Research and its Applications,” Vol. 2, W. Decler, L. Moens, H. Siegers, E. Jaspers and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar
- 24.S. M. Anderson, A. T. Bankier, B. G. Barreil, M. H. L. De Bruijn, A. R. Coulson, J. Drouin, I. C. Eperon, B. Nierlich, A. Roe, F. Sanger, P. H. Schreier, A. J. H. Smith, R. Staden and I. G. Young, Sequence and organization of human mitochondrial genome, Nature 290:457 (1981).PubMedCrossRefGoogle Scholar
- 26.D. R. Wolstenholme, D. O. Clary, J. L. MacFarlane, S. A. Wahleithener and L. Nilcox, Organization and evolution of invertebrate mitochondrial genomes, in: “Artemia Research and its Applications,” Vol. 2, W. Decleir, L. Moens, H. Siegers, E. Jaspers and P. Sorgeloos, eds., Universa Press, Wetteren (1987).Google Scholar
- 28.R. M. Schwartz and M. O. Dayhoff, “Cytochromes Atlas of Protein Sequence and Structure,” Vol. 5, Suppl 3, National Biomedical Foundation, Washington (1978).Google Scholar
- 29.M. S. Gardiner, “The Biology of Invertebrates,” McGraw-Hill, New York, (1972).Google Scholar