Kinetic and Thermodynamic Parameters of Artemia Ribosome Subunit Interactions: The Effects of Polyamines
Virtually all eucaryotic cells contain significant amounts of the polyamines— spermine, spermidine, and putrescine. The physiological function of these amines is not well understood at the molecular level, although recent studies have demonstrated that polyamines are required for cellular growth and differentiation and that the concentration within the cell is highly regulated[1,2]. In quiescent cells polyamines are present in very small amounts, although polyamine activity can be dramatically increased within a few hours of exposure to trophic stimuli. The pathway for polyamine biosynthesis has been determined, detailed descriptions are given in several recent reviews[4,5].
KeywordsRibosomal Subunit Brine Shrimp Association Reaction Polyamine Concentration Subunit Association
Unable to display preview. Download preview PDF.
- 1.U. Bachrach, Effect on growth processes, in: “Function of Naturally Occurring Polyamines”, Academic Press, New York (1973).Google Scholar
- 2.D. H. Russell, Polyamines in growth — normal and neoplastic, in: “Polyamines in Normal and Neoplastic Growth”, Raven Press, New York (1973).Google Scholar
- 3.G. Kramer and B. Hardesty, Ribosomes from Artemia cysts in cell-free translation of eukaryotic mRNA, in: “The Brine Shrimp Artemia”, G. Persoone, P. Sorgeloos, O. Roels, and E. Jaspers, eds., Universa Press, Wetteren, Belgium (1980).Google Scholar
- 4.A. E. Pegg and P. P. McCann, Polyamine metabolism and functions, Am. J. Physiol. 243:212 (1982).Google Scholar
- 8.C. L. Woodley and A. J. Wahba, The development of a translation system to examine mRNA and messenger ribonucleoproteins from Artemia, in: “The Brine Shrimp Artemia,” G. Persoone, P. Sorgeloos, O. Roels and E. Jaspers, eds., Universa Press, Wetteren (1980).Google Scholar
- 15.P. Nieuwenhuysen and J. Clauwaert, Physicochemical characterization of ribosomal particles from the eukaryote Artemia, J. Biol. Chem. 256:9623 (1981).Google Scholar
- 18.N. A. Draper and H. Smith, “Applied Regression Analysis”, Wiley, New York (1966).Google Scholar