Enzymes of Dinucleoside Oligophosphate Metabolism in Artemia Cysts and Larvae

  • Mark Prescott
  • Andrew D. Milne
  • Alexander G. McLennan
Part of the NATO ASI Series book series (NSSA, volume 174)


After the discovery in 1963 of the abundant nucleotide store of P1, P4 -diguanosine 5′-tetraphosphate, or P1, P4 -bis (5′-guanosyl) tetraphosphate (Gp4G) in Artemia embryos[1], it was logical to search for an enzyme capable of converting it into utilisable products. Such an enzyme, originally named diguanosine tetraphosphate asymmetrical-pyrophosphohydrolase (EC was soon discovered which cleaved Gp4G specifically to yield equimolar amounts of GTP and GMP[2]. Studies on the partially purified enzyme showed it to be primarily located in the soluble fraction of the cell, to have a molecular mass of 17,500 and to efficiently hydrolyse dinucleotides of the general structure Np4N, including Gp4G, Ap4A, Xp4X and Up4U[3,4]; it had little activity towards Gp3G which had also been found in significant quantities in embryos[2,5].


Brine Shrimp Physarum Polycephalum Artemia Salina Luminescence Assay Artemia Cyst 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Mark Prescott
    • 1
  • Andrew D. Milne
    • 1
  • Alexander G. McLennan
    • 1
  1. 1.Department of BiochemistryUniversity of LiverpoolLiverpoolUK

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