Multiple Protease Inhibitors in Artemia Cysts
Proteolysis in developing systems requires that intracellular proteases operate within certain physiological limits. Several factors appear to regulate intracellular protease activity including compartmentalization, membrane association, cofactor levels intracellular pH (pHi) and the presence of endogenous protease inhibitors . It now seems clear that proteolytic activity is required for yolk utilization, at least in embryos of amphibia  and crustacea [3,4], and perhaps in the unmasking of ribosomes [5,6] and mRNP particles  following fertilization or egg activation. However, in all cases it is imperative that proteolytic activity be controlled closely, regardless of its physiological purpose, so that inappropriate proteolysis doesn’t occur with resulting cytolysis and loss of embryo viability.
KeywordsBrine Shrimp Acid Protease Protamine Sulfate Intracellular Protease Artemia Cyst
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