An important key that has opened the door to an understanding of how living systems work is the ability to isolate elementary cellular components. This ability provides a powerful incentive to continually improve the procedures for the isolation of macromolecules. The scope of biological separations is overwhelmingly diverse. Even when the discussion is restricted to proteins, the problems range from the separation of macromolecules that strongly interact with each other to the resolution of molecules having only minute differences.
KeywordsIonic Strength Ammonium Sulfate Enzyme Purification Adsorption Chromatography Column Equilibration
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