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Estimation of Molecular Weight by Gel Filtration and Gel Electrophoresis

I. Mathematical Principles
  • David Rodbard
Part of the Biological Separations book series (BIOSEP)

Abstract

The most popular methods for estimation of molecular weights of proteins are gel filtration (GF) and polyacrylamide gel electrophoresis (PAGE) (see Appendix for a glossary of terms used in this chapter). These two approaches may employ aqueous media or denaturing solvents such as solutions of urea, guanidine, sodium dodecylsulfate (SDS), sodium decylsulfate (NaDS), or other detergents. The usual practice is to construct a “calibration curve” and to utilize linear interpolation for estimation of Stokes’ radius, molecular weight, or log molecular weight. This chapter provides a review of the various types of calibration curves available, with their theoretical and empirical bases, and attempts to provide a comprehensive and unified approach to analysis of data from gel filtration, PAGE, and SDS-PAGE.

Keywords

Effective Radius Axial Ratio Relative Mobility Sedimentation Coefficient Error Function Complement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • David Rodbard
    • 1
  1. 1.Reproduction Research Branch, National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaUSA

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