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Analytical and Preparative Polyacrylamide Gel Electrophoresis

An Objectively Defined Fractionation Route, Apparatus, and Procedures
  • A. Chrambach
  • D. Rodbard
  • T. M. Jovin
  • P. J. Svendsen
Part of the Biological Separations book series (BIOSEP)

Abstract

Biochemistry may be viewed as the attempt to fragment living matter to its component molecular species, and to elucidate the chemical reactions of these species first in two-component systems, then in ever more complex systems and finally to reconstitute life as the sum of its component chemical reactions. The first task, i.e., the isolation of nature’s building-block molecules, remains a formidable task. This chapter is concerned with the instrumentation and procedures available for isolation of any given charged species according to a generally applicable fractionation strategy.

Keywords

Polymerization Mixture Wall Adherence Buffer Reservoir Page Strategy Pore Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • A. Chrambach
    • 1
  • D. Rodbard
    • 1
  • T. M. Jovin
    • 2
  • P. J. Svendsen
    • 3
  1. 1.Reproduction Research Branch, National Institute of Child Health and Human DevelopmentNational Institutes of HealthBethesdaUSA
  2. 2.Abteilung Molekulare BiologieMax-Planck-Institut für Biophysikalische ChemieGöttingenGerman Federal Republic
  3. 3.The Protein LaboratoryUniversity of CopenhagenCopenhagenDenmark

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