Abstract
Gonadotropin-releasing hormone (GnRH) is a hypothalamic decapeptide, which stimulates gonadotropin release from the anterior pituitary. The first step in GnRH action (Conn et al., 1981b) is its recognition by specific binding sites (receptors) at the surface of gonadotrope cells. The interaction of GnRH with pituitary membrane preparations or cultured pituitary cells has been studied in detail by using radioiodinated, metabolically stable GnRH analogs (Clayton et al., 1978; Conne et al., 1979; Clayton and Catt, 1980, 1981; Marian and Conn, 1980; Naor et al., 1980; Hazum, 1981a; Marian et al., 1981; Meidan and Koch, 1981a). These studies have indicated the presence of a single class of high-affinity binding sites for both agonists and antagonists of GnRH in the pituitary. Recently, the presence of similar high affinity binding sites have been shown in the ovary and testis (reviewed by Clayton and Catt, 1981; Hsueh and Jones, 1981; Hazum, 1983). Characterization of the GnRH-receptor in the pituitary have indicated that the receptor is a glycoprotein which contains sialic acid residues (Hazum, 1982a) and that membrane phospholipids are involved in the interaction between the hormone and the receptor (Hazum et al., 1982a).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Adams, T.E., and Spies, H.G., 1981, Binding characteristics of gonadotropin-releasing hormone receptors throughout the estrous cycle of the hamster, Endocrinology, 108: 2245.
Blum, J.J., and Conn, P.M., 1982, Gonadotropin-releasing hormone stimulation of luteinizing hormone release: A ligand-receptor-effector model, Proc. Natl. Acad. Sci. U.S.A., 79: 7307.
Clayton, R.N., and Catt, K.J., 1980, Receptor-binding affinity of gonadotropin-releasing hormone analogs: Analysis by radioligand receptor assay, Endocrinology, 106: 1154.
Clayton, R.N., and Catt, K.J., 1981, Gonadotropin-releasing hormone receptors: Characterization, physiological regulation, and relationship to reproductive function, Endocrine Rev., 2: 186.
Clayton, R.N., Shakespear, R.A., and Marshall, J.C., 1978, LHRH binding to purified plasma membranes: Absence of adenylate cyclase activation., Mol. Cell. Endocrinol., 11: 63.
Clayton, R.N., Solano, A.R., Gracia-Vela, A., Dufau, M.I., and Catt, K.J., 1980, Regulation of pituitary receptors for gonadotropin-releasing hormone during the rat estrous cycle, Endocrinology, 107: 699.
Conn, P.M., and Hazum, E., 1981, LH release and GnRH-receptor internalization: Independent actions of GnRH, Endocrinology, 109: 2040.
Conn, P.M., Smith, R., and Rogers, D.C., 1981a, Stimulation of pituitary gonadotropin release does not require internalization of gonadotropin-releasing hormone, J. Biol. Chem., 256: 1098.
Conn, P.M., Marian, J., McMillian, M., Stern, J., Rogers, D., Hamby, M., Penna, A., and Grant, E., 1981b, Gonadotropin-releasing hormone action in the pituitary: A three step mechanism, Endocrine Rev., 2: 174.
Conn, P.M., Rogers, D.C., Stewart, J.M., Niedel, J., and Sheffield, T., 1982a, Conversion of gonadotropin-releasing hormone antagonist to an agonist, Nature, 296: 653.
Conn, P.M., Rogers, D.C., and McNeil, R., 1982b, Potency enhancement of a GnRH agonist: GnRH-receptor microaggregation stimulates gonadotropin release, Endocrinology, 111: 335.
Conne, B.S., Aubert, M.L., and Sizoneko, P.C., 1979, Quantification of pituitary receptor sites for LHRH: Use of superactive analog as a tracer, Biochem. Biophys. Res. Commun., 90: 1249.
Dacheux, F., 1981, Ultrastructural localization of gonadotropin-releasing hormone in porcine gonadotrophic cells, Cell Tissue Res., 216: 143.
Duello, T.M., and Nett, T.M., 1980, Uptake, localization, and retention of gonadotropin-releasing hormone and gonadotropin-releasing hormone analogs in rat gonadotrophs, Mol. Cell. Endocrinol., 19: 101.
Duello, T.M., Nett, T.M., and Farquhar, M.G., 1983, Fate of a gonadotropin-releasing hormone agonist internalized by rat pituitary gonadotrophs, Endocrinology, 112: 1.
Gregory, H., Taylor, C.L., and Hopkins, C.R., 1982, Luteinizing hormone release from dissociated pituitary cells by dimerization of occupied LHRH receptors, Nature, 300: 269.
Hazum, E., 1981a, Some characteristics of GnRH-receptors in rat pituitary membranes: Differences between an agonist and an antagonist, Mol. Cell. Endocrinol., 23: 275.
Hazum, E., 1981b, Photo-affinity inactivation of gonadotropin-releasing hormone receptors, FEBS Lett., 128: 111.
Hazum, E., 1981c, Photoaffinity labeling of luteinizing hormone releasing hormone receptors of rat pituitary membrane preparations, Endocrinology, 109: 1281.
Hazum, E., 1982a, GnRH-receptor of rat pituitary is a glycoprotein: Differential effect of neuraminidase and lectins on agonists and antagonists binding, Mol. Cell. Endocrinol., 26: 217.
Hazum, E., 1982b, Receptor regulation by hormones: Relevance to secretion and other biological functions, in: “Cellular Regulation of Secretion and Release”, P.M. Conn, ed., Academic Press, New-York.
Hazum, E., 1983, Nature of the GnRH receptors in the ovary, in: “Regulation of Target Cell Responsiveness” K.W. MoKerns, ed.. Plenum Press, New-York.
Hazum, E., and Keinan, D., 1982, Photoaffinity labeling of pituitary gonadotropin releasing hormone receptors during the rat estrous cycle, Biochem. Biophys. Res. Commun., 107: 695.
Hazum, E., and Keinan, D., 1983a, Covalent linking of photoreactive gonadotropin-releasing hormone to gonadotropes produces a prolonged signal, Proc. Natl. Acad. Sci. U.S.A., 80: 1902.
Hazum, E., and Keinan, D., 1983b, Gonadotropin releasing hormone receptors: photoaffinity labeling with an antagonist, Biochem. Biophys. Res. Commun., 110: 116.
Hazum, E., and Nimrod, A., 1982, Photoaffinity labeling and fluorescence distribution studies of gonadotropin-releasing hormone receptors in ovarian granulosa cells, Proc. Natl. Acad. Sci. U.S.A., 79: 1747.
Hazum, E., Cuatrecasas, P., Marian, J., and Conn, P.M., 1980, Receptor-mediated internalization of gonadotropin-releasing hormone by pituitary gonadotropes, Proc. Natl. Acad. Sci. U.S.A., 77: 6692.
Hazum, E., Fridkin, M., Baran, T., and Koch, Y., 1981, Degradation of gonadotropin-releasing hormone by anterior pituitary enzymes, FEBS Lett., 127: 273.
Hazum, E., Garritsen, A., and Keinan, D., 1982a, Role of lipids in gonadotropin releasing hormone agonist and antagonist binding to rat pituitary, Biochem. Biophys. Res. Commun., 105: 8.
Hazum, E., Meidan, R., Keinan, D., Okon, E., Koch, Y., Lindner, H.R., and Amsterdam, A., 1982b, A novel method for localization of gonadotropin releasing hormone receptors, Endocrinolol, 111: 2135.
Hazum, E., Liscovitch, M., Koch, Y., and Amsterdam, A., 1983a, Distribution of gonadotropin releasing hormone (GnRH) receptors in pituitary cells, 65th Ann. Meet. Endocrine Soc., San Antonio, Abs. 386.
Hazum, E., Meidan, R., Liscovitch, M., Keinan, D., Lindner, H.R., and Koch, Y., 1983b, Receptor-mediated internalization of LHRH antagonists by pituitary cells, Mol. Cell. Endocrinol., 30: 291.
Hopkins, C.R., and Gregory, H., 1977, Topographical localization of the receptors for luteinizing hormone-releasing hormone on the surface of dissociated pituitary cells, J. Cell Biol., 75: 528.
Hsueh, A.J.W., and Jones, P.B.C., 1981, Extrapituitary actions of gonadotropin-releasing hormone, Endocrine Rev., 2: 437.
Koch, Y., Baram, T., Hazum, E., and Fridkin, M., 1977, Resistance to enzymic degradation of LHRH analogues possessing increased biological activity, Biochem. Biophys. Res. Commun., 74: 488.
Marian, J., and Conn, P.M., 1980, The calcium requirement in GnRHstimulated LH release is not mediated through a specific action on receptor binding, Life Sci., 27: 87.
Marian, J., Cooper, R.L., and Conn, P.M., 1981, Regulation of the rat pituitary gonadotropin-releasing hormone receptor, Mol. Pharmacol., 19: 399.
Meidan, R., and Koch, Y., 1981a, Binding of luteinizing-hormone releasing hormone analogues to dispersed pituitary cells, Life Sci., 28: 1961.
Meidan, R., and Koch, Y., 1981b, Variations in luteinizing hormone-releasing hormone receptors in pituitary cells from immature and mature cycling female rats, FEBS Lett., 132: 114.
Naor, Z., Clayton, R.N., and Catt, K.J., 1980, Characterization of GnRH receptors in cultured rat pituitary cells, Endocrinology, 107: 1144.
Naor, Z., Atlas, A., Clayton, R.N., Forman, D.S., Amsterdam, A., and Catt, K.J., 1981, Interaction of fluorescent gonadotropinreleasing hormone with receptors in cultured pituitary cells, J. Biol. Chem., 256: 3049.
Savoy-Moore, B.T., Schwartz, N.B., Duncan, J.A., and Marshall, J.C., 1980, Pituitary gonadotropin-releasing hormone receptors during the rat estrous cycle, Science, 209: 942.
Sternberger, L.A., and Petrali, J.P., 1975, Quantitative immunocytochemistry of pituitary receptors for luteinizing hormone-releasing hormone, Cell Tissue Res., 162: 141.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1984 Springer Science+Business Media New York
About this chapter
Cite this chapter
Hazum, E. (1984). GnRH Receptors: Identification, Localization and Implications for Biological Function. In: McKerns, K.W., Naor, Z. (eds) Hormonal Control of the Hypothalamo-Pituitary-Gonadal Axis. Biochemical Endocrinology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9960-5_9
Download citation
DOI: https://doi.org/10.1007/978-1-4684-9960-5_9
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-9962-9
Online ISBN: 978-1-4684-9960-5
eBook Packages: Springer Book Archive