Molecular Heterogeneity of Luteinizing Hormone-Releasing Hormone
A number of studies purporting to have shown non-ribosomal biosynthesis of LH-RH and thereby excluding the possibility of ribosomally biosynthesised prohormonal forms.
The assumption that LH-RH was confined to the CNS and thus unlikely to be present in other tissues in a modified form.
Immunological and low resolution chromatographic studies demonstrating that LH-RH in the hypothalamus of nonmammalian vertebrates was identical to the mammalian peptide. This conclusion of a lack of interspecific differences in LH-RH structure in vertebrates was supported by the demonstration that synthetic mammalian LH-RH was biologically active in a wide range of mammalian species and in nonmammalian vertebrates (1).
KeywordsLuteinizing Hormone Molecular Heterogeneity Substantial Biological Activity High Molecular Weight Form Nonmammalian Vertebrate
Unable to display preview. Download preview PDF.
- 4.R.P. Millar, P. Denniss, C. Tobler, J.C. King, A.V. Schally, and A. Arimura, Presumptive prohormonal forms of hypothalamic peptide hormones, in “Cell biology of hypothalamic neurosecretion”, J.D. Vincent, and C. Kordon, eds., Centre National de la Recherche Scientifique 280, Bordeaux, pp. 487 (1978).Google Scholar
- 5.R.P. Millar, I. Wegener, and A.V. Schally, Putative prohormone of luteinizing hormone-releasing hormone, in “Neuropeptides: biochemical and physiological studies”, R.P. Millar, ed., Churchill-Livingstone, New York, pp. 111 (1981).Google Scholar
- 11.R.P. Millar, and C. Tobler, Structural and functional differences in pineal and hypothalamic luteinizing hormone-releasing hormone, in “Neuropeptides: biochemical and physiological studies”, R.P. Millar, ed., Churchill-Livingstone, New York, pp. 263 (1981).Google Scholar
- 12.R.P. Millar, P. Denniss, C. Tobler, and R.B. Symington, Immunological, biochemical and functional differences in pineal and hypothalamic luteinizing hormone-releasing hormone, in “Pineal function”, C.D. Matthews, and R.F. Seamark, eds., Elsevier/North-Holland Biomedical Press, Amsterdam, pp. 151 (1981).Google Scholar
- 14.S. Pavel, The mechanism of action of vasotocin in the mammalian brain, in “The pineal gland of vertebrates including man. Progress in brain research vol. 52”, J. Ariens Kappers, and P. Pevet, eds., Elsevier/North-Holland Biomedical Press, Amsterdam, pp. 445 (1979).Google Scholar
- 15.R.P. Millar, J.A. King, I. Wegener, C. Tobler, C. Dutlow, R.W. Roeske, W.A. Day, J.E. Rivier, W.W. Vale, and P. Licht, Molecular evolution of vertebrate luteinizing hormone-releasing hormone, in “ Neuronal Communications”, B. Meyer, and S. Kramer, eds., Balkema Press, Cape Town, (1983) in press.Google Scholar
- 19.C. Turkelson, T. Kenjo, and A. Arimura, Effects of an LHRH agonist in rat Leydig cell culture and purification of a testicular LHRH-like substance, 65th Annual Meeting of the Endocrine Society, abstract no. 373 (1983).Google Scholar
- 29.J.A. King, and R.P. Millar, Structure of chicken hypothalamic luteinizing hormone-releasing hormone. I. Structural determination on partially purified material, J. Biol. Chem. 257: 10722 (1982).Google Scholar
- 31.J.A. King, and R.P. Millar, Structure of avian hypothalamic gonadotrophin-releasing hormone, S. Afr. J. Sci. 78: 124 (1982).Google Scholar
- 33.J.A. King, and R.P. Millar, Phylogeny of vertebrate luteinizing hormone-releasing hormone and somatostatin, in “Neuropeptides: biochemical and physiological studies”, R.P. Millar, ed., Churchill-Livingstone, New York, pp. 217 (1981).Google Scholar
- 34.J. Rivier, C. Rivier, D. Branton, R. Millar, J. Spiess, and W. Vale, HPLC purification of ovine CRF, rat extrahypothalamic brain somatostatin and frog brain GnRH, in “ Peptides: synthesis-structure-function”, Proc. Seventh American Peptide Symposium, D.H. Rich, and E. Gross, eds., Pierce Chemical Company, Illinois, pp. 771 (1981).Google Scholar
- 41.R.P. Millar, and J.A. King, Synthesis, luteinizing hormone releasing activity, and receptor binding of chicken hypothalamic luteinizing hormone-releasing hormone, Endocrinology (in press).Google Scholar
- 43.J. Sandow, W. Konig, R. Geiger, R. Uhmann, and W. von Rechenberg, Structure-activity relationships in the LH-RH molecule, in “Control of ovulation”, D.B. Crighton, N.B. Haynes, G.R. Foxcroft, and G.E. Lamming, eds., Butterworths, London, pp. 49 (1978).Google Scholar
- 44.P. Licht, Evolutionary and functional aspects of pituitary gonadotropins in the green turtle, Chelonia mydas, Amer. Zool. 20: 561 (1980).Google Scholar
- 45.P. Licht, R. Millar, J.A. King, B.R. McCreery, M.T. Mendonca, A. Bona-Gallo, and B. Lofts, Effects of chicken and mammalian gonadotropin-releasing hormones (GnRH) on in vivo pituitary gonadotropin release in amphibians and reptiles, Gen. Comp. Endocrinol. (in press).Google Scholar
- 46.I.P. Callard, and V. Lance, The control of reptilian follicular cycles, in “Reproduction and evolution”, J.H. Calaby, and C.H. Tyndale-Biscoe, eds, Australian Acad. Sci., Canberra City, pp. 199 (1977).Google Scholar
- 51.R.E. Peter, Serum gonadotropin levels in mature male goldfish in response to lutypizing ormone-releasing hormone (LH-RH) and des-Gly -ID-Ala)-LH-RH ethylamide, Can. J. Zool. 58: 1100 (1980).Google Scholar