Secretion and Action of Steroids in the Luteal Cell

  • Geula Gibori
  • M. Iqbal Khan
  • Rajagopala Sridaran
  • Y-D. Ida Chen
  • Salman Azhar
  • Mrinalini C. Rao
  • Pondicherry Jayatilak
  • J. Randy Gruber
Part of the Biochemical Endocrinology book series (BIOEND)


The corpus luteum (CL) is a transient endocrine gland that synthesizes the steroid(s) necessary for ovum implantation and development of both placenta and fetus. In some species, such as primate and rat, the CL secretes both progesterone (P) and estradiol (E). However, luteal production of steroids is required for fetal survival throughout pregnancy in rats, while the CL is necessary only during early pregnancy in primates.


Corpus Luteum Lipoprotein Receptor Theca Cell Luteal Cell Ovarian Vein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Alloiteau, J.J., and Mayer, G., 1967, Problems concernant la formation, le maintien et la regression des corps jaunes chez le rat, Arch. Anat. Microsc. Morphol. Exp., 56: 189.PubMedGoogle Scholar
  2. Azhar, S., Chen, Y-D. I., and Reaven, G. M., 1983, Stimulation of lipoprotein receptors and role of lipoprotein and cellular cholesterol during gonadotropin induced desensitization of steroidogenic response in luteinized rat ovary, J. Biol. Chem., 258: 3735.PubMedGoogle Scholar
  3. Azhar, S., and Menon, K.M.J., 1981, Receptor-mediated gonadotropin action in the ovary. Rat luteal cells preferentially utilize and are acutely dependent upon the plasma lipoprotein-supplied sterols in gonadotropins-stimulated steroid production, J. Biol. Chem., 256: 6518.Google Scholar
  4. Basuray, R., and Gibori, G., 1980, Luteotropic action of the decidual tissue in the pregnant rat, Biol. Reprod., 23: 507.PubMedCrossRefGoogle Scholar
  5. Basuray, R., Jaffe, R., and Gibori, G., 1983, Role of decidualluteotropin and prolactin in the control of luteal cell receptor for estradiol, Biol. Reprod., 28: 551.PubMedCrossRefGoogle Scholar
  6. Beg, Z. H., and Brewer, H.B., Jr., 1982, Modulation of rat liver 3-hydroxy-3-methylglutaryl-CoA reductase activity by reversible phosphorylation, Fed. Proc., 41: 2634.PubMedGoogle Scholar
  7. Behrman, H.R., and Armstrong, D., 1969, Cholesterol esterase stimulation by luteinizing hormone in luteinized rat ovaries, Endocrinology, 85: 474.PubMedCrossRefGoogle Scholar
  8. Behrman, H.R., Luborsky-Moore, J.L., Pang, C.Y., Wright, K., and Dorflinger, L.J., 1979, Mechanisms of PGF2a action in functional luteolysis, in: “Ovarian Follicular and Corpus Luteum Function”, C. P. Channing, J. Marsh, and W. A. Sadler, eds., Plenum Press, New York.Google Scholar
  9. Bender, E.M., Miller, J.B., Possley, R.M., and Keyes, P.L., 1978, Steroidogenic effect of 170-estradiol in the rabbit: Stimulation of progesterone synthesis in prematurely regressing corpora lutea, Endocrinology, 103: 1937.PubMedCrossRefGoogle Scholar
  10. Blank, M.S., Dufau, M.L., and Friesen, H.G., 1979, Demonstration of potent gonadotropin-like biological activity in the serum of rats during mid-pregnancy, Life Sci., 92: 1527.Google Scholar
  11. Bogovich, K., and Richards, J.S., 1982, Androgen biosynthesis in developing ovarian follicles: Evidence that luteinizing hormone regulates thecal 17a-hydroxylase and C 17,20-lyase activities, Endocrinology, 111: 120.CrossRefGoogle Scholar
  12. Brown, M.S., Goldstein, J.L., and Dietschy, J.F.M, 1979, Active and inactive forms of 3-hydroxy-3 methylglutaryl coenzyme a reductase in the liver of the rat, J. Biol. Chem., 254: 5144.PubMedGoogle Scholar
  13. Bruot, B.C., Wiest, W.G., and Collins, D.C., 1982, Effect of low density and high density lipoproteins on progesterone secretion by dispersed corpora luteal cells from rats treated with aminopyrazolo-(3,4-d) pyrimidine, Endocrinology, 110: 1572.PubMedCrossRefGoogle Scholar
  14. Carr, B.R., and Simpson, E.R., 1981, Lipoprotein utilization and cholesterol synthesis by the human fetal adrenal gland, Endocrine Reviews, 2: 306.PubMedCrossRefGoogle Scholar
  15. Chait, A., Bierman, E.L., and Albers, J. J., 1978, Regulatory role of insulin in the degradation of low density lipoprotein by cultured human skin fibroblasts, Biochim. Biophys. Acta., 529: 292.PubMedCrossRefGoogle Scholar
  16. Chait, A., Bierman, E.L., and Albers, J. J., 1979, Regulatory role of triiodothyronine in the degradation of low density lipoprotein by cultured human skin fibroblasts, J. Clin Endocrinol. Metab. 48: 887.PubMedCrossRefGoogle Scholar
  17. Chao, Y.S., Windier, E.E., Chen, G.C., and Havel, R.J., 1979, Hepatic catabolism of rat and human lipoprotein in rats treated with 17a-ethinyl estradiol, J. Biol. Chem., 254: 11360.PubMedGoogle Scholar
  18. Elbaum, D.J., and Keyes, P.L., 1976, Synthesis of 17ß-estradiol by isolated ovarian tissues of the pregnant rat: Aromatization in the corpus luteum, Endocrinology, 99: 573.PubMedGoogle Scholar
  19. Fillios, L.C., Kaplan, R., Marlin, R.S., and Stare, F.J., 1958, Some aspects of the gonadal regulation of cholesterol metabolism, Am. J. Physiol., 193: 47.PubMedGoogle Scholar
  20. Fortune, J.E., and Armstrong, D.T., 1978, Hormonal control of 17ß-estradiol biosynthesis in proestrous rat follicles: Estradiol production by isolated theca verus granulosa, Endocrinology, 102: 227.PubMedCrossRefGoogle Scholar
  21. Gibori, G., 1979, Steroidal regulation of luteal tissue function in pregnant rat, Endocrinology, 104: A585.Google Scholar
  22. Gibori, G., Antczak, E., and Rothchild, I., 1977, The role of estrogen in the regulation of luteal progesterone secretion in the rat after day 12 of pregnancy, Endocrinology, 100: 1483.PubMedCrossRefGoogle Scholar
  23. Gibori, G., Basuray, R., and McReynolds, B., 1981, Luteotropic role of the decidual tissue in the rat: Dependency on intraluteal estradiol, Endocrinology, 108: 2060.PubMedCrossRefGoogle Scholar
  24. Gibori, G., and Keyes, P.L., 1978, Role of intraluteal estrogen in the regulation of the rat corpus luteum during pregnancy, Endocrinology, 102: 1176.PubMedCrossRefGoogle Scholar
  25. Gibori, G., and Keyes, P.L., 1980, Luteotropic role of estrogen in early pregnancy in the rat, Endocrinology, 106: 1584.PubMedCrossRefGoogle Scholar
  26. Gibori, G., Keyes, P.L., and Richards, J.S., 1978, A role for intraluteal estrogen in the mediation of LH action on the rat corpus luteum during pregnancy, Endocrinology, 103: 162.PubMedCrossRefGoogle Scholar
  27. Gibori, G., and Kraicer, P.F., 1973, Conversion of testosterone to estrogen by isolated corpora lutea of pregnancy in the rat, Biol. Reprod, 9: 309.PubMedGoogle Scholar
  28. Gibori, G., and Richards, J.S., 1978, Dissociation of two distinct luteotropic effects of prolactin: Regulation of luteinizing hormone-receptor content and progesterone secretion during pregnancy, Endocrinology, 102: 767.PubMedCrossRefGoogle Scholar
  29. Gibori, G., Richards, J.S., and Keyes, P.L., 1979, Synergistic effect of prolactin and estradiol in the luteotropic process in the pregnant rat: Regulation of estradiol receptor by prolactin, Biol. Reprod., 21: 419.PubMedCrossRefGoogle Scholar
  30. Gibori, G., Rothchild, I., Pepe, G.J., Morishige, W.K., and Lam, P., 1974, Luteotrophic action of decidual tissue in the rat, Endocrinology, 95: 1113.PubMedCrossRefGoogle Scholar
  31. Gibori, G., and Sridaran, R., 1981, Sites of androgen and estradiol production in the second half of pregnancy in the rat, Biol. Reprod., 24: 249.PubMedCrossRefGoogle Scholar
  32. Gibori, G., Sridaran, R., and Basuray, R., 1982, Control of aromatase activity in luteal and non luteal cells of pregnant rats, Endocrinology, 111: 781.PubMedCrossRefGoogle Scholar
  33. Goldsmith, L.T., De La Cruz, J.L., Weiss, G., and Castracane, V. D., 1982, Steroid effects on relaxin secretion in the rat, Biol. Reprod., 27: 886.PubMedCrossRefGoogle Scholar
  34. Hunzicker-Dunn, M., and Birnbaumer, L., 1976, Adenyl cyclase activities in ovarian tissues. IV. Gonadotropin-induced desensitization of the luteal adenyl cyclase throughout pregnancy and pseudopregnancy in the rabbit and rat, Endocrinology, 99: 211.PubMedCrossRefGoogle Scholar
  35. Ingebritsen, T.S., Geelen, M.S.H., Parker, R.A., Evenson, K. J., and Gibson, D. M., 1979, Modulation of hydroxymethyl-CoA reductase activity, reductase kinase activity, and cholesterol synthesis in rat hepatocytes in response to insulin and glucagon, J. Biol. Chem., 254: 9986.PubMedGoogle Scholar
  36. Kalison, B., and Gibori, G., 1983, Role of prolactin in hCG stimulation of luteal cell production of testosterone and estradiol in the rat. in: “Factors Regulating Ovarian Function” G.S. Greenwald, and P.F. Terranova, eds., Raven Press, New York.Google Scholar
  37. Kelly, P.A., Shiu, R.P.C., Robertson, M.C., and Friesen, H.G., 1975, Characterization of rat chorionic mammotropin, Endocrinology, 96: 1187.PubMedCrossRefGoogle Scholar
  38. Keiner, K., Malinow, R., and Anderson, W., 1977, Effect of estradiol-17ß on cholesterol metabolism in the rat: A study using a deuterium label and mass spectrometry, Steroids, 29: 1.CrossRefGoogle Scholar
  39. Khan, M.I., Glaser, L., and Gibori, G., 1983, Reactivation of regressing corpora lutea by estradiol: Dependence on rat placental lactogen, Fed. Proc., 42: A143.Google Scholar
  40. Kovanen, P.T., Brown, M.S., and Goldstein, J.L., 1979, Increased binding of low density lipoprotein to liver membranes from rats treated with 17a-ethinyl estradiol, J. Biol. Chem., 245: 11367.Google Scholar
  41. MacDonald, G.J., Armstrong, D.T., and Greep, R.O., 1966, Stimulation of estrogen secretion from normal rat corpora lutea by luteinizing hormone, Endocrinology, 79: 289.PubMedCrossRefGoogle Scholar
  42. Madhwa Raj, H.G., and Moudgal, N.R., 1970, Hormonal control of gestation in the intact rat, Endocrinology, 84: 874.Google Scholar
  43. Maizels, E.T., and Jungmann, R.A., 1982, Ca2+-dependent phosphorylation of rat ovary proteins, Biochem. Biophys. Res. Comm., 107: 32.PubMedCrossRefGoogle Scholar
  44. McNamara, B.C., Booth, R., and Stansfield, D.A., 1981, Evidence for an essential role for high density lipoprotein in progesterone synthesis by rat corpus luteum, FEBS Lett., 134: 79.PubMedCrossRefGoogle Scholar
  45. Morishige, W.K., and Rothchild, I., 1974, Temporal aspects of the regulation of corpus luteum function by luteinizing hormone, prolactin and placental luteotróphin during the first half of pregnancy in the rat, Endocrinology, 95: 260.PubMedCrossRefGoogle Scholar
  46. Nakamura, Y., and Ichikawa, S., 1978, Effect of the placental luteotropin and estrogen on the growth of artificially formed secondary corpora lutea of pregnancy in rats, Biol. Reprod., 19: 1014.PubMedCrossRefGoogle Scholar
  47. Ochiai, A., and Rothchild, I., 1981, The relation between conceptus number and the luteotropic effect of estrogen in rats after hypophysectomy and hysterectomy on day 12 of pregnancy, Endocrinology, 109: 1111.CrossRefGoogle Scholar
  48. Ohashi, M., Carr, B.R., and Simpson, E.R., 1982, Lipoprotein-binding sites in human corpus luteum membrane fractions, Endocrinology, 110: 1477.PubMedCrossRefGoogle Scholar
  49. Palfrey, H.C., 1983, Presence in many mammalian tissues of an identical major cytosolic substrate (Mr 100,000) for calmodulindependent protein kinase, FEBS Lett., 157: 183.PubMedCrossRefGoogle Scholar
  50. Rodway, R.G., and Garris, D.R., 1982, Potentiation by prolactin of the luteotrophic effect of estradiol in the pregnant rat, Acta. Endocrinol., 101: 287.PubMedGoogle Scholar
  51. Schuler, L.A., Scavo, L., Kirsch, T.M., Flickinger, G. L., and Strauss, J. F., III, 1979, Regulation of de novo biosynthesis of cholesterol and progestogens and formation of cholesteryl ester in rat corpus luteum by exogenous sterol, J. Biol. Chem., 245: 8662.Google Scholar
  52. Smith, M.S., McLean, B.K., and Neill, J.D., 1976, Prolactin the initial luteotropic stimulus of pseudopregnancy in the rat, Endocrinology, 98: 1370.PubMedCrossRefGoogle Scholar
  53. Sridaran, R., Basuray, R., and Gibori, G., 1981, Source and regulation of testosterone secretion in pregnant and pseudopregnant rats, Endocrinology, 108: 855.PubMedCrossRefGoogle Scholar
  54. Sridaran, R., Hunzicker-Dunn, M., and Gibori, G., 1983, Testosterone stimulation of progesterone synthesis by gonadotropin desensitized corpora lutea, Endocrinology, 112: 610.PubMedCrossRefGoogle Scholar
  55. Takayama, M., and Greenwald, G.S., 1973, Direct luteotropic action of estrogen in the hypophysectomized hysterectomized rat, Endocrinology, 92: 1405.PubMedCrossRefGoogle Scholar
  56. Taya, K., and Greenwald, G.S., 1981, In vivo and in vitro ovarian steroidogenesis in the pregnant rat, Biol. Reprod., 25: 683.PubMedCrossRefGoogle Scholar
  57. Wilkinson, R. F., Anderson, E., and Aalberg, J., 1976, Cytological observations of dissociated rat corpus luteum, J. Ultrastruct., 57: 168.CrossRefGoogle Scholar
  58. Windier, T., Kovanen, P.T., Chao, Y.S., Brown, M.S., Havel, R.J., and Goldstein, J.L., 1980, The estradiol-stimulated lipoprotein receptor of rat liver, J. Biol. Chem., 255: 10464.Google Scholar
  59. Witte, L. D., and Cormicelli, J. A., 1980, Platelet derived growth factor stimulates low density lipoprotein receptor activity in cultured human fibroblasts, Proc. Natl. Acad. Sci. USA, 77: 5962.PubMedCrossRefGoogle Scholar
  60. Veldhuis, J.D., and Klase, P.A., 1982, Role of calcium ions in the stimulatory actions of luteinizing hormone in isolated ovarian cells: Studies with divalent cation ionophores, Biochem. Biophys. Res. Commun., 104: 603.PubMedCrossRefGoogle Scholar
  61. Zmigrod, A., and Lindner, H. R., 1972, Oestrogen biosynthesis by the rat ovary in early pregnancy, Acta. Endocrinol. (Copenh), 69: 127.Google Scholar

Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Geula Gibori
    • 1
  • M. Iqbal Khan
    • 1
  • Rajagopala Sridaran
    • 1
  • Y-D. Ida Chen
    • 2
  • Salman Azhar
    • 2
  • Mrinalini C. Rao
    • 1
  • Pondicherry Jayatilak
    • 1
  • J. Randy Gruber
    • 1
  1. 1.Department of Physiology and Biophysics, College of MedicineUniversity of IllinoisChicagoUSA
  2. 2.Department of MedicineStanford University School of MedicinePalo AltoUSA

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