Fluidity of Gonadotropin Storage in Cycling Female Rats

  • Gwen V. Childs
Part of the Biochemical Endocrinology book series (BIOEND)


Hormone storage in gonadotropes has been the subject of a debate for over a decade. The cytophysiological evidence for non-parallel secretion of LH and FSH agreed with the early morphological evidence that there were two types of gonadotropes (Farquhar and Rinehart, 1954; Barnes, 1962). Workers postulated that each might be responsible for the secretion of only one gonadotropin (Barnes, 1962; Kurosumi and Oota, 1968). However, in the early 1970’s, immunocytochemists showed that both hormones were present in most of the same cells (Nakane, 1970; Phifer, et al., 1972; Tougard, et al., 1971, 1973). Furthermore, the two morphological cell types could not be distinguished on the basis of their gonadotropin content. During the subsequent 10 years a number of studies reported results from serially sectioned pituitaries that included a variety of species. These results showed collectively that most gonadotropes contained both hormones (multihormonal) (Tixier-Vidal et al., 1975; Herbert, 1975; Batten and Hopkins, 1978; Hopkins et al., 1981; Smith and Keefer, 1982). A number of these studies also reported that there were cells that contained only FSH or LH (monohormonal) (Nakane, 1970; Moriarty, 1976a, b; Pelletier, et al., 1976; El Etreby and Fath El Bab, 1977; Purandare, et al., 1978; Bugnon et al., 1977; Dacheux, 1978, 1980; Girod, et al., 1980; Fellmann et al., 1982; Jansen, 1982; Childs, et al., 1980, 1981, 1982a, b, 1983a, b; Dada, et al., 1983).


Estrous Cycle Pituitary Cell Centrifugal Elutriation Serial Field Initial Cell Suspension 
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Copyright information

© Springer Science+Business Media New York 1984

Authors and Affiliations

  • Gwen V. Childs
    • 1
  1. 1.Department of AnatomyThe University of Texas Medical BranchGalvestonUSA

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