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Extraction and Quantitation of B-6 Vitamers from Animal Tissues and Human Plasma: A Preliminary Study

  • Joseph T. Vanderslice
  • Catherine E. Maire
  • Gary R. Beecher

Abstract

Reports from recent conferences on vitamin B-6 metabolism indicate that it would be most useful to identify and quantitate all forms of vitamin B-6 in animal tissue and physiological fluids (1,2). Enzyme or microbiological assays by themselves do not yield information on the individual species, but chemical assays, particularly those involving high performance liquid chromatography (HPLC), do have this potential (3,4). The problem with chemical assays is that a suitable extraction procedure must be found which quantitatively extracts the compound(s) of interest from complex samples, prevents decomposition or metabolism during the extraction, and is compatible with the analytical HPLC systems.

Keywords

High Performance Liquid Chromatography High Performance Liquid Chromatography Human Plasma Pyridoxal Phosphate Microbiological Assay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Joseph T. Vanderslice
    • 1
  • Catherine E. Maire
    • 1
  • Gary R. Beecher
    • 1
  1. 1.United States Department of AgricultureBeltsville Human Nutrition Research Center Human NutritionBeltsvilleUSA

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