Extraction and Quantitation of B-6 Vitamers from Animal Tissues and Human Plasma: A Preliminary Study

  • Joseph T. Vanderslice
  • Catherine E. Maire
  • Gary R. Beecher


Reports from recent conferences on vitamin B-6 metabolism indicate that it would be most useful to identify and quantitate all forms of vitamin B-6 in animal tissue and physiological fluids (1,2). Enzyme or microbiological assays by themselves do not yield information on the individual species, but chemical assays, particularly those involving high performance liquid chromatography (HPLC), do have this potential (3,4). The problem with chemical assays is that a suitable extraction procedure must be found which quantitatively extracts the compound(s) of interest from complex samples, prevents decomposition or metabolism during the extraction, and is compatible with the analytical HPLC systems.


High Performance Liquid Chromatography High Performance Liquid Chromatography Human Plasma Pyridoxal Phosphate Microbiological Assay 
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Literature Cited

  1. 1.
    National Research Council (1978) Human vitamin B-6 requirements. Nat. Acad. Sci., Washington, DC.Google Scholar
  2. 2.
    Tryfiates, G. P., ed. (1980) Vitamin B-6 metabolism and role in growth. Food and Nutrition Press, Inc., Westport, CT.Google Scholar
  3. 3.
    Vanderslice, J. T., Stewart, K. K. & Yarmas, M. M. (1979) Liquid chromatographic separation and quantification of B-6 vitamers and their metabolite, pyridoxic acid. J. Chromatogr. 176, 280–285.CrossRefGoogle Scholar
  4. 4.
    Vanderslice, J. T. & Maire, C. E. (1980) Liquid chromatographic separation and quantification of B-6 vitamers at plasma concentration levels. J. Chromatogr. 196, 176–179.CrossRefGoogle Scholar
  5. 5.
    Vanderslice, J. T., Maire, C. E., Doherty, R. F. & Beecher, G. R. (1980) Sulfosalicylic acid as an extraction agent for vitamin B-6 in food. J. Agric. Food Chem. (In press).Google Scholar
  6. 6.
    Vanderslice, J. T., Maire, C. E. & Beecher, G. R. (1980) B-6 vitamer analysis in human plasma by HPLC: a preliminary study. Am. J. Clin. Nutr. (In press).Google Scholar
  7. 7.
    Vanderslice, J. T., Maire, C. E. & Yakupkovic, J. E. (1980) Vitamin B-6 in ready-to-eat cereals: analysis by high performance liquid chromatography. J. Food Sci. (In press).Google Scholar
  8. 8.
    Hess, B. & Brand, K. (1963) Cell and tissue disintegration. In: Methods of Enzymatic Analysis (Bergmeyer, H. V., ed.), 2ndedition, pp. 396–409, Academic Press, New York.Google Scholar
  9. 9.
    Nordstrom, J. L., Rodwell, V. W. & Mitschelen, J. J. (1977) Interconversion of active and inactive forms of rat liver hydroxmethylglutaryl-CoA reductase. J. Biol. Chem. 252, 8924–8934.Google Scholar
  10. 10.
    Anderson, B. B. (1980) Red-cell metabolism of vitamin B-6. In: Vitamin B-6 Metabolism and Role in Growth (Tryfiates, G. P., ed.), pp. 53–84, Food and Nutrition Press, Inc., Westport, CT.Google Scholar
  11. 11.
    Lumeng, L. & Li, T.-K. (1980) Mammalian vitamin B-6 metabolism: regulatory role of protein-binding and the hydrolysis of pyridoxal 5’-phosphate in storage and transport. In: Vitamin B-6 Metabolism and Role in Growth (Tryfiates, G. P., ed.), pp. 27–51, Food and Nutrition Press, Westport, CT.Google Scholar
  12. 12.
    Peterson, E. A. & Sober, H. A. (1954) Preparation of crystalline phosphorylated derivatives of vitamin B-6. J. Am. Chem. Soc. 76, 169–175.CrossRefGoogle Scholar
  13. 13.
    Bain, J. A. & Williams, H. L. (1960) Concentrations of B-6 vitamers in tissues and tissue fluids. In: Inhibitions in the Nervous System and Gammaaminobutyric Acid (Baxter, C. F., Harreveld, A. V., Wiersma, C. A. G., Adley, W. R. & Killam, K. F., eds.), pp. 275–281, Pergamon Press, New York.Google Scholar
  14. 14.
    Thiele, V. F. & Brin, M. (1966) Chromatographic separation and microbiological assay of vitamin B-6 in tissues from normal and vitamin B-6-depleted rats. J. Nutr. 90, 347–353.Google Scholar
  15. 15.
    Sloger, M. S., Scholfield, L. G. & Reynolds, R. D. (1978) Loss of in vitro inactivation of rat liver tyrosine aminotransferase with dietary vitamin B-6 restriction. J. Nutr. 108, 1355–1360.Google Scholar
  16. 16.
    Polansky, M. M. & Toepfer, E. W. (1969) Vitamin B-6 components in some meats, fish, dairy products and commercial infant formulas. J. Agric. Food Chem. 17, 1394–1397.CrossRefGoogle Scholar
  17. 17.
    Lumeng, L. & Li, T.-K. (1978) Plasma content of B-6 vitamers and its relationship to hepatic vitamin B-6 metabolism. Fed. Proc. 37, 588.Google Scholar
  18. 18.
    Shane, B. (1978) Vitamin B-6 and blood. In: Human Vitamin B-6 Requirements, pp. 111–128, Nat. Acad. Sci., Washington, DC. National Research Council, Washington, DC.Google Scholar
  19. 19.
    Wachstein, M., Kellner, J. D. & Ortiz, J. M. (1960) Pyridoxal phosphate in plasma and leukocytes in patients with leukemia and other diseases. Proc. Soc. Exp. Biol. Med. 105, 563–566.Google Scholar
  20. 20.
    Hamfelt, A. (1962) A method of determiningpyridoxal phosphate in blood by decarboxylation of L-tyrosine-14C(U). Clin. Chim. Acta 7, 746–748.Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Joseph T. Vanderslice
    • 1
  • Catherine E. Maire
    • 1
  • Gary R. Beecher
    • 1
  1. 1.United States Department of AgricultureBeltsville Human Nutrition Research Center Human NutritionBeltsvilleUSA

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