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Microbiological Assay of Vitamin B-6 in Blood and Urine

  • Lorraine T. Miller
  • Margaret Edwards

Abstract

Although new, more precise techniques have been developed for the determination of vitamin B-6, the microbiological assay of this vitamin in blood and urine is still an important and valuable method. The assay organism used in our laboratory to measure vitamin B-6 is the yeast, Saccharomyces uvarum (formerly, S. carlsbergensis). Vitamin B-6 determination with this organism is simple, convenient, reproducible, and sensitive. In this paper, we will present the background for our choice of this organism and our procedure for determining plasma and urinary vitamin B-6.

Keywords

Hydrolysis Time Potassium Citrate Pyridoxal Phosphate Microbiological Assay Total Vitamin 
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Literature Cited

  1. 1.
    Storvick, C. A. & Peters, J. M. (1964) Methods for the determination of vitamin B-6 in biological materials. Vit. and Hormones 22, 833–854.CrossRefGoogle Scholar
  2. 2.
    Rabinowitz, J. C. & Snell, E. E. (1951) The nature of the requirement of Saccharomyces carlsbergensis for vitamin B-6. Arch. Biochem. Biophys. 33, 472–481.Google Scholar
  3. 3.
    Haskell, B. E. & Snell, E. E. (1970) Microbiological deter- mination of the vitamin B-6 group. In: Methods in Enzymology (McCormick, D. B. & Wright, L. D., eds.), vol. 18A, pp. 512519, Academic Press, New York.Google Scholar
  4. 4.
    Haskell, B. E. & Wallnofer, U. (1967) D-Alanine interference in microbiological assays of vitamin B-6 in human blood. Anal. Biochem. 19, 569–577.Google Scholar
  5. 5.
    Anderson, B. A., Peart, M. B. & Fulford-Jones, C. E. (1970) The measurement of serum pyridoxal by a microbiological assay using Lactobacillus casei. J. Clin. Pathol. 23, 232–242.CrossRefGoogle Scholar
  6. 6.
    Horwitz, W., ed. (1980) Official Methods of Analysis of the Assoc. Off. Anal. Chem., 13th ed., pp. 768–769, Washington, DC.Google Scholar
  7. 7.
    Rose, D. P. (1974) Assessment of tryptophan metabolism and vitamin B-6 nutrition in pregnancy and oral contraceptive users. In: Biochemistry of Women: Methods for Clinical Investigation (Curry, A. S. and Hewitt, J. V., ed.), pp. 317–349, C.R.C. Press, Inc., Cleveland, OH.Google Scholar
  8. 8.
    Lindberg, A. S. (1980) The effect of wheat bran on the bio-availability of vitamin B-6 in humans. M.S. Thesis, Oregon State University.Google Scholar
  9. 9.
    Wozenski, J. R., Leklem, J. E. & Miller, L. T. (1980) The metabolism of small doses of vitamin B-6 in men. J. Nutr. 110, 275–285.Google Scholar
  10. 10.
    Miller, L. T., Dow, M. J. & Kokkeler, S. C. (1978) Methionine metabolism and vitamin B-6 status in women using oral contraceptives. Am. J. Clin. Nutr. 31, 619–625.Google Scholar
  11. 11.
    Rabinowitz, J. C. & Snell, E. E. (1949) Vitamin B-6 group. XV. Urinary excretion of pyridoxal, pyridoxamine, pyridoxine, and 4-pyridoxic acid in human subjects. Proc. Soc. Exp. Biol. Med. 70, 235–240.Google Scholar

Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Lorraine T. Miller
    • 1
  • Margaret Edwards
    • 1
  1. 1.Department of Foods and NutritionOregon State UniversityCorvallisUSA

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