Microbiological Assay of Vitamin B-6 in Blood and Urine

  • Lorraine T. Miller
  • Margaret Edwards


Although new, more precise techniques have been developed for the determination of vitamin B-6, the microbiological assay of this vitamin in blood and urine is still an important and valuable method. The assay organism used in our laboratory to measure vitamin B-6 is the yeast, Saccharomyces uvarum (formerly, S. carlsbergensis). Vitamin B-6 determination with this organism is simple, convenient, reproducible, and sensitive. In this paper, we will present the background for our choice of this organism and our procedure for determining plasma and urinary vitamin B-6.


Hydrolysis Time Potassium Citrate Pyridoxal Phosphate Microbiological Assay Total Vitamin 
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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Lorraine T. Miller
    • 1
  • Margaret Edwards
    • 1
  1. 1.Department of Foods and NutritionOregon State UniversityCorvallisUSA

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