Microbiological Assay of Vitamin B-6 in Foods

  • Marilyn Polansky


In 1939, only a year after the isolation of vitamin B-6 and 5 years after its discovery, Schultz et al. (1) and Eakin et al.(2) reported that vitamin B-6 was effective in yeast growth stimulation and that this stimulation might be useful as a method for determination of vitamin B-6. In 1943, Atkin et al. (3) presented a microbiological method for determining pyridoxine (PN), the only known form at that time, using a strain of Saccharomyces carlsbergensis (culture 4228), now renamed Saccharomyces uvarum (ATCC 9080) by American Type Culture Collection. This yeast was especially selected for its specific response to pyridoxine. The initial yeast used in the yeast growth method for assay of vitamin B-6 was not specific. In a pyridoxine-free medium,S.uvarum grows very slightly, but when pyridoxine was added to the medium an extensive growth was observed. With a few exceptions the yeast method gave results that agreed reasonably well with results obtained by the rat growth method. This same year, Stokes, et al. (4) presented a method for determining vitamin B-6 by using a mold, Neurospora sitophila as the test organism. Also in 1943, Siegel et al. (5) reported modifications of the Williams yeast growth method for determination of pyridoxine in acid extracts of natural products.


Growth Response Microbiological Assay Total Vitamin Absorbent Cotton Yeast Method 


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Copyright information

© Springer Science+Business Media New York 1981

Authors and Affiliations

  • Marilyn Polansky
    • 1
  1. 1.United States Department of AgricultureBeltsville Human Nutrition Research CenterBeltsvilleUSA

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