Incidence and Sources of Mycoplasma Contamination: A Brief Review
In 1956, Robinson and colleagues (1) reported the first isolation of a mycoplasma from a contaminated cell culture. Subsequently, mycoplasmas have been shown to be common and bothersome contaminants capable of altering the activity of cells and affecting the results of study. Because many of the vaccines prepared for human use are produced in cell cultures and are subject to mycoplasma contamination, the Bureau of Biologics has maintained a continuing study for the past 18 years to examine various aspects of mycoplasma contamination. This report will review some of our findings and present a brief, updated status report on the incidence, prevalence and sources of mycoplasma contamination. The subject has been reviewed in detail elsewhere (2–5).
KeywordsPrimary Cell Culture Mycoplasma Contamination Mycoplasma Species Bovine Seron Swine Origin
Unable to display preview. Download preview PDF.
- 3.Barile, M. F. 1973. Mycoplasma contamination of cell cultures: Mycoplasma-virus-cell culture interactions, In: J. Fogh (Ed.) Contamination of CeZZ Cultures. Academic Press, New York, NY pp. 131–172.Google Scholar
- 4.Barile, M. F. 1974. General principles of isolation and detection of mycoplasmas. In: J. M. Bove and J. F. Duplan (Eds.) Les Mycoplasmes. Colloques INSERM, Paris 33: pp. 135–142.Google Scholar
- 5.Barile, M. F. 1977. Mycoplasma contamination of cell cultures: A status report. In: R. Action (Ed.) Cell Culture and Its Application. Academic Press, New York, NY.Google Scholar
- 6.Barile, M. F. 1962. Discussion: Detection and elimination of contaminating organisms. Bethesda, J. Nat. Cancer Institute, Monograph Series #7, pp. 50–53.Google Scholar
- 9.Barile, M. F., and R. A. DelGiudice. 1972. Isolation of mycoplasmas and their rapid identification by plate epiimmunofluorescence. In: Ciba Foundation Symposium. Pathogenic Mycoplasmas. Elsevier and North-Holland, Amsterdam. pp. 165–188.Google Scholar