Advertisement

Incidence and Sources of Mycoplasma Contamination: A Brief Review

  • Michael F. Barile
  • Hope E. Hopps
  • Marion W. Grabowski
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 3)

Abstract

In 1956, Robinson and colleagues (1) reported the first isolation of a mycoplasma from a contaminated cell culture. Subsequently, mycoplasmas have been shown to be common and bothersome contaminants capable of altering the activity of cells and affecting the results of study. Because many of the vaccines prepared for human use are produced in cell cultures and are subject to mycoplasma contamination, the Bureau of Biologics has maintained a continuing study for the past 18 years to examine various aspects of mycoplasma contamination. This report will review some of our findings and present a brief, updated status report on the incidence, prevalence and sources of mycoplasma contamination. The subject has been reviewed in detail elsewhere (2–5).

Keywords

Primary Cell Culture Mycoplasma Contamination Mycoplasma Species Bovine Seron Swine Origin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Robinson, L. B., R. B. Wichelhausen, and B. Roizman. 1956. Contamination of human cell cultures by pleuropneumonia-like organisms. Science 124: 1147–1148.PubMedCrossRefGoogle Scholar
  2. 2.
    Barile, M. F., R. A. DelGiudice, H. E. Hopps, M. W. Grabowski, and D. B. Riggs. 1973. Identification of Mycoplasma species isolated from contaminated cell cultures and commercial bovine sera. N. Y. Acad. Sci. 225: 251–264.CrossRefGoogle Scholar
  3. 3.
    Barile, M. F. 1973. Mycoplasma contamination of cell cultures: Mycoplasma-virus-cell culture interactions, In: J. Fogh (Ed.) Contamination of CeZZ Cultures. Academic Press, New York, NY pp. 131–172.Google Scholar
  4. 4.
    Barile, M. F. 1974. General principles of isolation and detection of mycoplasmas. In: J. M. Bove and J. F. Duplan (Eds.) Les Mycoplasmes. Colloques INSERM, Paris 33: pp. 135–142.Google Scholar
  5. 5.
    Barile, M. F. 1977. Mycoplasma contamination of cell cultures: A status report. In: R. Action (Ed.) Cell Culture and Its Application. Academic Press, New York, NY.Google Scholar
  6. 6.
    Barile, M. F. 1962. Discussion: Detection and elimination of contaminating organisms. Bethesda, J. Nat. Cancer Institute, Monograph Series #7, pp. 50–53.Google Scholar
  7. 7.
    Barile, M. F., W. F. Malizia, and D. Riggs. 1962. Incidence and detection of pleuropneumonia-like organisms in cell culture by fluorescent antibody and cultural procedures. J. Bacteriol. 84: 130–136.PubMedGoogle Scholar
  8. 8.
    Barile, M. F., and J. Kern. 1971. Isolation of Mycoplasma arginini from commercial bovine sera and its implication in contaminated cell cultures. Proc. Soc. Exp. Biol. Med. 138: 432–437.PubMedGoogle Scholar
  9. 9.
    Barile, M. F., and R. A. DelGiudice. 1972. Isolation of mycoplasmas and their rapid identification by plate epiimmunofluorescence. In: Ciba Foundation Symposium. Pathogenic Mycoplasmas. Elsevier and North-Holland, Amsterdam. pp. 165–188.Google Scholar
  10. 10.
    Hopps, H. E., B. C. Meyer, M. F. Barile, and R. A. DelGiudice. 1973. Problems concerning “non-cultivable” mycoplasma contaminants in tissue cultures. N. Y. Acad. Sci. 225: 265–276.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Michael F. Barile
    • 1
  • Hope E. Hopps
    • 1
  • Marion W. Grabowski
    • 1
  1. 1.Bureau of BiologicsFDABethesdaUSA

Personalised recommendations