Methods of Prevention, Control and Elimination of Mycoplasma Infection

  • Gerard J. McGarrity
  • Veronica Vanaman
  • Judith Sarama
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 3)


Mycoplasme infected cell cultures should not be used for experimental or diagnostic work. Many reports have been published in this text and elsewhere on the effects of mycoplasme infection on cultured cells. These reports represent only a small portion of the total effects of infection. In a particular infected culture, the possible simultaneous effects are numerous, including: alterations in nucleic and amino acid metabolism, depletion of nutrients from the medium, chromosomal damage, phenotypic alteration, enzyme induction, induction of interferon, and membrane changes. An alteration in one characteristic can induce additional changes in other characteristics. Aula and Nichols demonstrated that chromosome damage by mycoplasmes was caused by depletion of arginine from the medium (1). Therefore, the overall effect of infection can be the additive or synergistic total of all mycoplasmal effects. Some effects may not become immediately produced and may become apparent only after 10, 20 or more population doublings following infection. For these reasons, studies showing no apparent difference between infected and non-infected cultures as a rationale to use infected cultures should be viewed skeptically.


Infected Culture Mycoplasma Infection Sterility Test Airborne Bacterium Mycoplasma Species 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Gerard J. McGarrity
    • 1
  • Veronica Vanaman
    • 1
  • Judith Sarama
    • 1
  1. 1.Department of MicrobiologyInstitute for Medical ResearchCamdenUSA

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