Effects of Mycoplasmas on Lymphocyte Cell Cultures

  • Gerard J. McGarrity
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 3)


The use of human lymphocyte cultures for cytogenetic studies was reported by Chrustschoff and Berlin in 1935 (1). Because of difficulties in propagation and maintenance of lymphocyte cell cultures, work was limited. Normal leukocytes do not ordinarily divide in peripheral blood although some do have mitotic potential. Early studies were designed to determine what factors could activate this latent potential. Plasma concentration, oxygen tension and carbon dioxide had only minor effects. In 1955, Rigas and Osgood reported the characteristics of phytohemagglutinin (PHA), a mitogen present in the red kidney bean, Phaseolus vulgaris (2). This plant extract was originally used to agglutinate erythrocytes in obtaining leukocytes from whole blood, and was shown to induce mitosis in leukocytes.


Lymphocyte Culture Lymphocyte Transformation Maple Syrup Urine Disease Interferon Production Leukemoid Reaction 
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Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Gerard J. McGarrity
    • 1
  1. 1.Department of MicrobiologyInstitute for Medical ResearchCamdenUSA

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