Advertisement

Genetic Effects of Mycoplasma

  • Warren W. Nichols
Part of the Cellular Senescence and Somatic Cell Genetics book series (CSSCG, volume 3)

Abstract

In this paper I'll summarize the effects and the apparent effects of mycoplasma on chromosomes and genes. The best known genetic effects of mycoplasma are on chromosomes. This was first reported by Fogh and Fogh in 1965 (1). In this report chromosomes of the FL/amnion line had been followed for 9 years without appreciable change in number. When the cells were infected with mycoplasma there was a reduction in chromosome number and a rise in chromosome aberrations. The chromosome number gradually decreased from a range of 70–76 to 63–68. Chromosome aberrations included open breaks and stable and unstable rearrangements. These started during the first days after infection and developed slowly in mycoplasma-infected cultures. In subsequent work (2,3) it was demonstrated that after elimination of mycoplasma by aureomycin treatment the reduced chromosome number and stable rearrangements persisted, but unstable aberrations were reduced to pre-infection levels.

Keywords

Chromosome Aberration Chromosome Breakage Human Diploid Cell Hypoxanthine Phosphoribosyl Transferase Amniotic Fluid Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Fogh, J. and Fogh, H. Chromosome changes in PPLO-infected FL human amnion cells. Proc. Soc. Exptl. Biol. Med. 119: 233 - 238, 1965.Google Scholar
  2. 2.
    Fogh, J. and Fogh, H. Karyotypic changes in mycoplasma-modified lines of FL human amnion cells. Proc. Soc. Exptl. Biol. Med. 129: 944 - 950, 1968.Google Scholar
  3. 3.
    Fogh, J. and Fogh, H. Irreversibility of major chromosome changes in a mycoplasma-modified line of FL human amnion cells. Proc. Soc. Exptl. Biol. Med. 126: 67 - 74, 1967.Google Scholar
  4. 4.
    Paton, G.R., Jacobs, J.P. and Perkins, F.T. Chromosome changes in human diploid cell cultures infected with mycoplasma. Nature 207: 43 - 45, 1965.PubMedCrossRefGoogle Scholar
  5. 5.
    Aula, P. and Nichols, W.W. The cytogenetic effects of mycoplasma in human leukocyte cultures. Jrl. Cell. Physiol. 70: 281 - 290, 1967.CrossRefGoogle Scholar
  6. 6.
    Freed, J.J. and Schatz, S.A. Chromosome aberrations in cultured cells deprived of single essential amino acids. Exptl. Cell Res. 55: 393 - 409, 1969.PubMedCrossRefGoogle Scholar
  7. 7.
    Stanbridge, E., Önen, M., Perkins, F.T. and Hayflick, L. Karyological and morphological characteristics of human diploid cell strain WI-38 infected with mycoplasma. Exptl. Cell Res. 57: 397 - 410, 1969.PubMedCrossRefGoogle Scholar
  8. 8.
    Kundsin, R.B., Ampola, M., Streeter, S. and Neurath, P. Chromosomal aberrations induced by T strain mycoplasmas. Jrl. Med. Genetics 8: 181 - 187, 1971.CrossRefGoogle Scholar
  9. 9.
    Copperman, R. and Morton, H.E. Reversible inhibition of mitosis in lymphocyte cultures by non-viable mycoplasma. Proc. Soc. Exptl. Biol. Med. 123: 790 - 795, 1966.Google Scholar
  10. 10.
    Paton, G., Jacobs, J.P. and Perkins, F.T. The effect of mycoplasma on the karyology of normal cells. Ann. N.Y. Acad. Sci. 143: 626 - 627, 1967.PubMedCrossRefGoogle Scholar
  11. 11.
    Kuz'mina, S.V. Action of mycoplasmas on the chromosomal apparatus of mouse fibroblasts in tissue culture. Soviet Genetics 8: 126 - 127, 1972.PubMedGoogle Scholar
  12. 12.
    Schneider, E.L., Stanbridge, E.J., Epstein, C.J., Golbus, M., Abbo-Halbasch, G. and Rodgers, G. Mycoplasma contamination of cultured amniotic fluid cells: potential hazard to prenatal chromosomal diagnosis. Science 184: 477 - 480, 1974.PubMedCrossRefGoogle Scholar
  13. 13.
    Stanbridge, E. Mycoplasmas and cell cultures. Bacteriological Rev. 35: 206 - 227, 1971.Google Scholar
  14. 14.
    Nardone, R.M., Todd, J., Gonzalez, P. and Gaffney, E.V. Nucleoside incorporation into strain L cells: inhibition by pleuropneumonia-like organisms. Science 139: 1100 - 1101, 1965.CrossRefGoogle Scholar
  15. 15.
    Clive, D., Flamm, W.G. and Patterson, J.B. Specific-locus mutational assay systems for mouse lymphoma cells. In: CHEMICAL MUTAGENS, Vol. 3, edt. A. Hollaender, pp. 79 - 103, Plenum Press, 1973.Google Scholar
  16. 16.
    Fogh, J. and Fogh, H. A method for direct demonstration of pleuropneumonia-like organisms in cultured cells. Proc. Soc. Exptl. Biol. Med. 117: 809 - 901, 1964.Google Scholar

Copyright information

© Springer Science+Business Media New York 1978

Authors and Affiliations

  • Warren W. Nichols
    • 1
  1. 1.Institute for Medical ResearchCamdenUK

Personalised recommendations