Intracellular Recording from the Giant Synapse of the Squid

  • Theodore H. Bullock
  • Susumu Hagiwara


Several authors have described intracellular recording from the post-junctional cell in synaptic regions, for example, in the neuromuscular junction (Fatt and Katz, 1951, 1953), in the brain and spinal cord (Brock, Coombs, and Eccles, 1952; Woodbury and Patton, 1952; Tasaki, Polley, and Orrego, 1954; Tasaki, Hagiwara, and Watanabe, 1954; Araki and Otani, 1955; Coombs, Eccles, and Fatt, 1955; Frank and Fuortes, 1955, 1956), in the sympathetic ganglion cell (R. Eccles, 1955), the electroplaque of electric fish (Albe-Fessard and Buser,. 1952; Albe-Fessard and Chagas, 1954; Altamirano, Coates, and Grundfest, 1955), and in the large monopolar ganglion cells of certain invertebrates (Tauc, 1955; Arvanitaki and Chalazonitis, 1956; Hagiwara and Watanabe, 1956). Only in the nerve-muscle junction of the frog and the electroplaque of the eel, however, have the records been obtained from a region of the postjunctional cell known to be near the prefiber ending and only in the former is the junction supplied with but one input fiber. The giant synapse in the stellate ganglion of the squid between the giant fibers of the second and the third order offers a particularly favorable object for such recordings. Especially valuable is the opportunity to place microelectrodes internally in the presynaptic fiber close to the ending as well as in the postsynaptic unit, close to the junction. This preparation was studied some years ago with macroelectrodes on the surface of the ganglion (Bullock, 1948). A preliminary account of the present observations has appeared (Bullock and Hagiwara, 1955).


Postsynaptic Membrane Stellate Ganglion Synaptic Potential Post Fiber Synaptic Delay 
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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Theodore H. Bullock
    • 1
  • Susumu Hagiwara
    • 2
  1. 1.Department of ZoologyUniversity of CaliforniaUSA
  2. 2.Los Angeles, California,. and The Marine Biological LaboratoryWoods HoleUSA

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