Cell Biology of Serum-Free Mouse Embryo (SFME) Cells

  • Masayoshi Iio
  • Yoko Fuke
  • David W. Barnes
Conference paper
Part of the Serono Symposia, USA book series (SERONOSYMP)


Mouse embryo cells cultured at moderate densities under conventional conditions with serum as a medium supplement undergo a period of proliferation followed by growth crisis and the emergence of aneuploid immortalized cultures (1, 2). We derived mouse embryo cell lines that do not show crisis or aneuploidy when grown for more than 10 times the number of generations that can be achieved precrisis by conventional means (2–13). These lines are maintained in a serum-free medium formulation and are markedly growth inhibited by serum.


Epidermal Growth Factor Receptor Epidermal Growth Factor Nerve Growth Factor Glial Fibrillary Acidic Protein Population Doubling 
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Copyright information

© Springer-Verlag New York, Inc. 1993

Authors and Affiliations

  • Masayoshi Iio
  • Yoko Fuke
  • David W. Barnes

There are no affiliations available

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