Gas Chromatography of Vitamin B6 and Other Vitamins

  • Walter Korytnyk


Although a number of methods are available for the assay of vitamins [1-5], gas chromatography, where applicable, may offer unique advantages in speed of separation, sensitivity, and convenience in quantitation. The recently introduced combination of gas chromatography with mass spectrometry [6] can be expected to provide a powerful tool for analytical studies in this field, such as those concerned with the metabolic fates of vitamins and their analogs. The present chapter embodies an attempt to indicate the potential usefulness of gas chromatography in vitamin research in general and in the study of vitamin B6 in particular.


Acetyl Derivative Trimethylsilyl Derivative Acid Lactone Antimony Trichloride Diethylene Glycol Succinate 
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  1. 1.
    R. Strohecker and H.M. Henning, Vitamin Assay, Verlag Chemie, 1965.Google Scholar
  2. 2.
    E. Heftmann, Chromatography, Reinhold, New York, 1960, pp. 645Google Scholar
  3. 2a.
    E. Heftmann, Chromatography, Reinhold, New York, 1960, pp. 473Google Scholar
  4. 2b.
    E. Heftmann, Chromatography, Reinhold, New York, 1960, pp. 597.Google Scholar
  5. 3.
    C.A. Storvick and J.M. Peters, Vitamins Hormones, 22:833, 1964.CrossRefGoogle Scholar
  6. 4.
    C.A. Storvick, E.M. Benson, M.A. Edwards, and M.J. Woodring, Methods Biochem. Anal. 12:183, 1964.Google Scholar
  7. 5.
    E.W. Toepfer and M.M. Polansky, Vitamins Hormones 22:825, 1964.CrossRefGoogle Scholar
  8. 6.
    S.W. Downer, in: L.R. Mattick and H.A. Szymanski., eds., Lectures on Gas Chromatography, Plenum Press, New York, 1965, p. 189.Google Scholar
  9. 7.
    E.E. Snell, Vitamins Hormones 16:77, 1958.CrossRefGoogle Scholar
  10. 8.
    S. Shaltiel, E.H. Fischer, and J.L. Hedrick, Biochemistry 5:2109 1966.CrossRefGoogle Scholar
  11. 9.
    B.E. Haskell and E.E. Snell, Arch. Biochem. Biophys. 112:494, 1965.CrossRefGoogle Scholar
  12. 10.
    E.E. Snell et al., Chemical and Biological Aspects of Pyridoxal Catalysis, Macmillan, New York, 1963.Google Scholar
  13. 11.
    W. Korytnyk, J. Med. Chem. 8:112, 1965CrossRefGoogle Scholar
  14. 11a.
    W. Korytnyk and B. Paul, J. Heterocyclic Chem. 2:144, 1965. References to earlier papers in the series are given in these two papers.CrossRefGoogle Scholar
  15. 12.
    W. Korytnyk, G. Fricke, and B. Paul, Anal. Biochem., 17:66, 1966.CrossRefGoogle Scholar
  16. 13.
    W. Korytnyk and W. Wiedeman, J. Chem. Soc., p. 2531, 1962.Google Scholar
  17. 14.
    R. Kuhn and G. Wendt, Ber. 71:780, 1938.Google Scholar
  18. 15.
    W. Korytnyk, J. Org. Chem. 27:3721, 1962.CrossRefGoogle Scholar
  19. 16.
    W. Korytnyk and B. Paul, Tetrahedron Letters, No. 8, p. 777, 1966.CrossRefGoogle Scholar
  20. 17.
    After the principal study cited in the present paper was completed, a preliminary report on the gas chromatography of some acetyl derivatives of vitamin B-compounds appeared: A.R. Prosser and A.J. Sheppard, Federation Proc. 25:669, 1966.Google Scholar
  21. 18.
    T. Hashizume and Y. Sasaki, Anal. Biochem. 15:199, 1966.CrossRefGoogle Scholar
  22. 19.
    C.C. Sweeley, R. Bentley, M. Makita, and W.W. Wells, J.Am. Chem. Soc. 85:2497, 1963.CrossRefGoogle Scholar
  23. 20.
    S. Friedman and M.L. Kaufman, Anal. Chem. 38:144, 1966.CrossRefGoogle Scholar
  24. 21.
    B. Paul and W. Korytnyk, Abstracts of the 152nd Meeting of the American Chemical Society, September, 1966, p. 63.Google Scholar
  25. 22.
    D.C. De Jongh, S.C. Perricone, and W. Korytnyk, J. Am. Chem. Soc. 88:1233, 1966, and unpublished results.CrossRefGoogle Scholar
  26. 23.
    H. Kubiezkova, V. Rezl, and N.Kucharczyk, Abstracts, J. Gas Chromat. 1, No. 4, 1963.Google Scholar
  27. 24.
    R.N. Roberts, J.A. Johnston, and W.B. Fuhr, Anal. Biochem. 10:282, 1965.CrossRefGoogle Scholar
  28. 25.
    K.K. Carroll and D.C. Herting, J. Am. Oil Chem. Soc. 41:473, 1964.CrossRefGoogle Scholar
  29. 26.
    H. Ziffer, W. J.A. Van den Heuvel, E.O.A. Haahti, and E.C. Horning, J. Am. Chem. Soc. 82:6411, 1960.CrossRefGoogle Scholar
  30. 27.
    P.P. Nair, C. Bucana, S. de Leon, and D.A. Turner, Anal. Chem. 37:631, 1965.CrossRefGoogle Scholar
  31. 28.
    N. Nicolaides, J. Chromat. 4:496, 1960.CrossRefGoogle Scholar
  32. 29.
    P.W. Wilson, E. Kodicek, and V.H. Booth, Biochem. J. 84:524, 1962.Google Scholar
  33. 30.
    P.P. Nair and D.A. Turner, J. Am. Oil Chem. Soc. 40:353, 1963.CrossRefGoogle Scholar
  34. 31.
    J.G. Bieri and E.L. Andrews, Iowa State J. Sci. 38:3, 1963.Google Scholar
  35. 32.
    W.R. Supina, in: H.A. Szymanski, ed., Biomedical Applications of Gas Chromatography, Plenum Press, New York, 1964, p. 271.Google Scholar
  36. 33.
    T.K. Murray, K.C. Day, and E. Kodicek, Biochem. J. 98:293, 1966, 96:29P, 1965.Google Scholar
  37. 34.
    L.V. Avioli and S.W. Lee, Anal. Biochem. 16:163, 1966.CrossRefGoogle Scholar

Copyright information

© Plenum Press 1967

Authors and Affiliations

  • Walter Korytnyk
    • 1
  1. 1.Department of Experimental TherapeuticsRoswell Park Memorial InstituteBuffaloUSA

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