Elastic Tissue Histochemistry
The object of our project has been to isolate, purify and characterize elastin from normal, fetal and pathological tissues and to relate the chemical findings to the morphological description of the tissue. Verhoeff’s iron haematoxylin technique (1) was adopted as the standard stain as it has reliably given a good differentiation between elastin and other connective tissue elements. However, it has not given entirely satisfactory results in pathological sections or in fetal tissue. To overcome some of these difficulties we have developed a fluorescent technique and have experimented with the Oxytalan fiber stain developed by Lillie & Fullmer (2).
KeywordsAldehyde Group Fetal Tissue Elastin Fiber Pathological Section Vocal Process
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