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Elastic Tissue Histochemistry

  • D. A. Keith
  • M. A. Paz
  • P. M. Gallop
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)

Abstract

The object of our project has been to isolate, purify and characterize elastin from normal, fetal and pathological tissues and to relate the chemical findings to the morphological description of the tissue. Verhoeff’s iron haematoxylin technique (1) was adopted as the standard stain as it has reliably given a good differentiation between elastin and other connective tissue elements. However, it has not given entirely satisfactory results in pathological sections or in fetal tissue. To overcome some of these difficulties we have developed a fluorescent technique and have experimented with the Oxytalan fiber stain developed by Lillie & Fullmer (2).

Keywords

Aldehyde Group Fetal Tissue Elastin Fiber Pathological Section Vocal Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Verhoeff, F. H. (1908) J. Amer. Med. Assn. 50 876.CrossRefGoogle Scholar
  2. 2.
    Fullmer, H. M. & Lillie, R. D. (1958) Histochem Cytochem. 6 425–430.CrossRefGoogle Scholar
  3. 3.
    Fullmer, H. M., Sheetz, J. H. and Narkates, A. J. (1974) J. Oral Path. 3 291–316.PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • D. A. Keith
    • 1
  • M. A. Paz
    • 1
  • P. M. Gallop
    • 1
  1. 1.Departments of Biological Chemistry, Orthopaedic Surgery and Oral Biology, Harvard Medical SchoolHarvard School of Dental Medicine and Children’s Hospital Medical CenterBostonUSA

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