Abstract
Insoluble elastin has been isolated from bovine 1igamentum nuchae by treatment with guanidine and dithiothreitol followed by digestion with collagenase, purified by affinity chromatography. The preparation was subjected to both wide- and low-angle X-ray analysis. The wide-angle diffraction patterns of relaxed and stretched specimens showed only two broad diffraction rings, corresponding to spacings of 4.5 and 9.3 Å. No significant reflections were visible in the low-angle diffraction pattern of unstretched specimens, but on stretching an equatorial reflection was produced, corresponding to spacings of between 45 and 50 Å.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Hoeve, C.A.J. and Flory, P.J. (1958) J. Am. Chem Soc. 80, 6523–6526.
Hoeve, C.A.J. and Flory, P.J. (1974) Biopolymers 13, 677–686.
Volpin, D. and Ciferri, A. (1970) Nature 225, 382.
Mistrali, F., Volpin, D., Garibaldo, G.B. and Ciferri, A. (1971) J. Phys. Chem. 75, 142–149.
Ross, R. and Bornstein, P. (1969) J. Cell Biol. 40, 366–381.
Gotte, L. and Serafini-Fracassini, A. (1963) J. Atheroscler. Res. 3, 247–251.
Gotte, L., Meneghelli, V., and Castellani, A. (1965) in Structure and Function of Connective and Skeletal Tissue (Fitton Jackson, S., Partridge, S.M., Harkness, R.D. and Tristram, G.R., eds), pp. 93–101, Butterworths, London.
Serafini-Fracassini, A. and Tristram, G.R. (1966) Proc. R. Soc. Edin. 69B, 334–344.
Serafini-Fracassini, A., Field, J.M., Spina, M, Stephens, W.G.S. and Delf, B. (1976) J. Mol. Biol. 100, 73–84.
Gotte, L., Giro, M.G., Volpin, D. and Home, R.W. (1974) J. Ultrastruct. Res. 46, 23–33.
Serafini-Fracassini, A., Field, J.M., Rodger, G.W. and Spina, M. (1975) Biochim. Biophys. Acta 386, 80–86.
Spina, M., Garbin, G., Field, J.M. and Serafini-Fracassini, A. (1975) Biochim. Biophys. Acta 400, 162–166.
Gotte, L., Mammi, M. and Pezzin, G. (1968) Symposium on Fibrous Proteins, pp. 236–245, (Crewther, W.G., ed), Butterworths, London.
Fraser, R.D.B., and MacRae, T.P. (1973) Conformation in Fibrous Proteins and Related Synthetic Polypeptides, p. 471, Academic Press, New York.
Partridge, S.M. (1968) in Symposium on Fibrous Proteins (Crewther, W.G., ed), pp. 246–264, Butterworths, London.
Gray, W.R., Sandberg, L.B. and Foster, J.A. (1973) Nature 246, 461–466.
Ayer, P.A. (1964) in International Review of Connective Tissue Research (Hall, D.A. ed), Vol. 2, pp. 45–53, Academic Press, New York.
Gotte, L., Mammi, M. and Pezzin, G. (1972) Connect. Tissue Res. 1, 61–67.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1977 Plenum Press, New York
About this chapter
Cite this chapter
Serafini-Fracassini, A., Field, J.M., Delf, B. (1977). X-Ray Analysis of Enzymically Purified Elastin from Bovine Ligamentum Nuchae. In: Sandberg, L.B., Gray, W.R., Franzblau, C. (eds) Elastin and Elastic Tissue. Advances in Experimental Medicine and Biology, vol 79. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9093-0_58
Download citation
DOI: https://doi.org/10.1007/978-1-4684-9093-0_58
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-9095-4
Online ISBN: 978-1-4684-9093-0
eBook Packages: Springer Book Archive