The Kinetics of Elastolysis as A Probe of Elastin
The purification of amorphous elastin from elastic tissue has generally required the use of harsh chemical procedures. It would not be surprising that these procedures alter some of the properties of the amorphous elastin (1). Several authors have reported enhancement of the rate of enzymatic hydrolysis of elastin concomitant with harsher methods of purification (2, 3, 4). We have confirmed these observations and also report striking differences in the effect of sodium dodecyl sulfate on the enhancement of elastolysis. Kagan et al. have reported a two-to three-fold stimulation of elastolysis by sodium dodecyl sulfate in 30mM NaCl and a twenty-to fifty-fold stimulation of elastolysis in 154mM NaCl (5). However, we find that Lansing treated elastin exhibits no significant stimulation in 30mM NaCl and an attenuated level of stimulation in 154mM NaCl (Table 1). The absence of sodium dodecyl sulfate stimulation of elastolysis in 30mM NaCl solution is observed with Lansing prepared elastin substrate from calf ligament, calf aorta and dog lung.
KeywordsSodium Dodecyl Sulfate Elastic Tissue Pancreatic Elastase Porcine Pancreatic Elastase Alanine Content
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