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Quantitative NH2 Terminal Evaluation of Elastin as A Measure of Polypeptide Integrity

  • V. L. Alvarez
  • L. B. Sandberg
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)

Abstract

The quality of a given elastin preparation has been a matter of growing concern to those of us involved in isolating this fiber from complicated tissues such as the lung. Amino acid analysis is not a good index of quality. When we speak of quality we specifically refer to integrity of the polypeptide chains of the elastin which has been isolated. In this paper we wish to present a method that appears promising for the evaluation of such quality. It is a method which looks at the free NH2 terminal amino acids in purified elastin, identifies and quantitates them in a relatively short period of time. The need for the method arose after we had applied various “non-degradative” techniques in isolating elastin from porcine lung. The analyses in all these preparations look very similar and yet we were quite certain that the integrity of the polypeptide chains varied considerably. We had specifically focused on a method described by Serafini-Fracassini, et al. (1), which involved the use of highly purified collagenase to rid the elastin of collagen contaminants.

Keywords

Amino Acid Analysis Cyanogen Bromide Degradation Index High Pressure Liquid Chro Isoleucine Leucine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • V. L. Alvarez
    • 1
  • L. B. Sandberg
    • 1
  1. 1.Department of PathologyUniversity of Utah Medical CenterSalt Lake CityUSA

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