Abstract
Lysyl oxidase had been purified to near homogeneity from bovine aorta and bovine ligamentum nuchae employing a modification of methods described by Harris et al., and Stassen and his colleagues. The aortic enzyme gives rise to at least three peaks and the ligament enzyme resolves into at least four peaks upon chromatography on DEAE cellulose. The molecular weight of each peak of both enzymes is approximately 30,000 daltons in sodium dodecyl sulfate. The aortic enzyme aggregates to species with molecular weights varying from approximately 60,000 to 1,000,000 daltons upon dialysis out of urea into phosphate-buffered saline. Temperature studies reveal that lysyl oxidase is stable to temperatures as high as 80°C, although the assay optimum is 52°C. Studies in progress suggest the temperature dependency of assay may reflect conformational changes in the elastin substrate.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Pinnell, S.R. and Martin, G.R. (1968) Proc. Nat. Acad. Sci. U.S. 61, 708.
Franzblau, C. in “Comprehensive Biochemistry”, Florkin, M. and Stotz, E.H., eds., Vol. 26C, Elsevier, Amsterdam, 1971, p. 659.
Gallop, P.M., Blumenfeld, O.O. and Seifter, S. (1972) Ann. Rev. Biochem. 41, 617.
Siegel, R.D., Pinnell, S.R. and Martin, G.R. (1970) Biochemistry9, 4486.
Stassen, F.L.H. (1976) Biochim. Biophys. Acta438, 49.
Kagan, H.M., Hewitt, N.A., Salcedo, L.L. and Franzblau, C. (1974) Biochim. Biophys. Acta365, 223.
Harris, E.D., Gonnerman, W.A., Savage, J.E. and O’Dell, B.L. (1973) Biochim. Biophys. Acta341, 332.
Vidal, G.P., Shieh, J.J. and Yasunobu, K.T. (1975) Biochem. Biophys. Res. Commun. 64, 989.
Cuatrecasas, P. in “Biochemical Aspects of Reactions on Solid Supports”, Stark, G.R., ed., 1971, Academic Press, N.Y., p. 79.
Weber, K. and Osborne, M. (1969) J. Biol.Chem. 244, 4406.
Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) J. Biol.Chem. 193, 265.
Siegel, R.C. (1974) Proc. Nat. Acad. Sci. U.S. 71, 4826.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1977 Plenum Press, New York
About this chapter
Cite this chapter
Jordan, R.E., Milbury, P., Sullivan, K.A., Trackman, P.C., Kagan, H.M. (1977). Studies on Lysyl Oxidase of Bovine Ligamentum Nuchae and Bovine Aorta. In: Sandberg, L.B., Gray, W.R., Franzblau, C. (eds) Elastin and Elastic Tissue. Advances in Experimental Medicine and Biology, vol 79. Springer, Boston, MA. https://doi.org/10.1007/978-1-4684-9093-0_44
Download citation
DOI: https://doi.org/10.1007/978-1-4684-9093-0_44
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4684-9095-4
Online ISBN: 978-1-4684-9093-0
eBook Packages: Springer Book Archive