Some Investigations of Elastin Biosynthesis In Vitro Using an Immunoprecipitant

  • Bryan Sykes
  • Susan Hawker
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)


The examination of early stages of elastin biosynthesis has been hampered by the lack of a reliable handle for the protein. The production of a hyperimmune serum in sheep which forms specific immunoprecipitates with salt-soluble elastin (1) is a potentially useful tool for this type of investigation. Its use in the measurement of elastin 1n tissue extracts has already been described (2), but here we report how this facility has been extended to the study of elastin biosynthesis in vitro.


Neutral Salt Phenyl Methyl Sulphonyl Fluoride Phenyl Methyl Sulphonyl Hyperimmune Serum Molecular Size Distribution 
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  1. 1.
    Sykes, B.C. and Chidlow, J.W., (1974) FEBS Lett, 47, 272.CrossRefGoogle Scholar
  2. 2.
    Sykes, B.C. and Partridge, S.M., (1974) Biochem. J., 141, 567.PubMedGoogle Scholar
  3. 3.
    Sykes, B.C. and Bailey, A.J., (1971) Biochem. Biophys. Res. Commun., 43, 340.Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Bryan Sykes
    • 1
  • Susan Hawker
    • 1
  1. 1.Department of Orthopaedic SurgeryOxford UniversityUSA

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