Detection and Estimation of Newly Synthesized Soluble Elastin Using a Hydroxyproline Label

  • F. W. Keeley
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)


In recent years, as more has been learned about the occurrence and character of soluble elastin, it has become possible to begin looking at the biochemistry of synthesis and secretion of this protein in ways which were previously not feasible. One of the prerequisites for this sort of investigation is a reliable and, if possible, convenient assay system by which the protein can be detected and estimated. Newly synthesized, radio-actively labelled soluble elastin has been identified in several ways; by the addition of unlabelled carrier and subsequent purification of the protein (1), by its ability to serve as a substrate for lysyl oxidase (2) and by electrophoresis on Polyacrylamide gels in the presence of sodium dodecylsulphate (3). The technique that we have been using, and which we think offers some advantages over others, is a variation on the last of these three methods.


Sodium Dodecylsulphate Lysyl Oxidase Longe Incubation Time Increase Incubation Time Entire Incubation Period 
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  1. 1.
    Smith, D.W. and Carnes, W.H. (1973) J. Biol. Chem. 248, 8157–8161.PubMedGoogle Scholar
  2. 2.
    Narayanan, A.S., Page, R.C. and Martin, G.R. (1974) Biochim. Biophys. Acta 351, 126–132.PubMedCrossRefGoogle Scholar
  3. 3.
    Murphy, L., Harsch, M., Mori, T. and Rosenbloom, J. (1972) FEBS Letters 21, 113–117.PubMedCrossRefGoogle Scholar
  4. 4.
    Keeley, F.W. (1976) Connective Tissue Res. 4, 195–205.CrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • F. W. Keeley
    • 1
  1. 1.Research Institute, Hospital for Sick Children and Department of BiochemistryUniversity of TorontoTorontoCanada

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