Studies on the Major Fucosylated Glycoprotein Released into the Medium by Cultured Human Skin Fibroblasts

  • Christopher H. J. Sear
  • Michael E. Grant
  • David S. Jackson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)


Electrophoretic analyses of the proteins synthesized by cultured human skin fibroblasts demonstrated that the major component released into the medium is a high molecular weight fucosylated glycoprotein (MFGP). Gel filtration chromatography under denaturing and reducing conditions indicated that MFGP has a molecular weight of approx. 250,000; but appears to behave as a disulphide-linked dimer when unreduced. MFGP is further distinguished as the major labelled macromolecule in the medium after incubation of fibroblasts with [35s]cysteine. The role of this glycoprotein is unknown but it bears a striking resemblance to a presumptive structural glycoprotein recently shown to be secreted by arterial smooth muscle cells in culture, and may also be related to a group of much studied cell surface glycoproteins.


Skin Fibroblast Human Skin Fibroblast Arterial Smooth Muscle Cell Agarose Column Smooth Muscle Cell Culture 
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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Christopher H. J. Sear
    • 1
  • Michael E. Grant
    • 1
  • David S. Jackson
    • 1
  1. 1.Department of Medical BiochemistryUniversity of Manchester, Medical SchoolManchesterEngland

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