Crosslinking Profiles of Elastin from Anatomically Defined Regions of the Lung
Elastin was isolated and purified by a procedure which sequentially removed lipids, collagen, structural glycoproteins and the microfibrillar proteins. Cross-linking profiles were obtained by column chromatography either after reduction with 3[H]NaBH4 or after reaction with 14[C]NaCN and NH3. Examination of the crosslinking profiles of the elastins from various tissue regions revealed differences in the type, distribution and quality of crosslinks.
KeywordsAlkali Treatment Structural Glycoprotein Aorta Elastin Calf Lung Unreduced Sample
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