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Comparison of the Elastolytic Effects of Human Leukocyte Elastase and Porcine Pancreatic Elastase

  • Robert M. Senior
  • David R. Bielefeld
  • Barry C. Starcher
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)

Abstract

Porcine and bovine elastins were digested by human leukocyte elastase and porcine pancreatic elastase. The enzymes showed similarities in the extent to which they digested elastin and the pattern and quantitative distribution of N-terminal amino acids in the digests. However, fingerprints of the digests showed differences between the products of leukocyte elastase and pancreatic elastase. Each enzyme produced its characteristic fingerprint irrespective of whether the elastin substrate was obtained from ligament, pleura or lung parenchyma. The enzymes also digested tropoelastin differently. The results suggest that leukocyte elastase and pancreatic elastase should not be considered interchangeable in experimental models of tissue injury.

Keywords

Leukocyte Elastase Pancreatic Elastase Porcine Pancreatic Elastase Dansylated Amino Acid Human Leukocyte Elastase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Robert M. Senior
    • 1
  • David R. Bielefeld
    • 1
  • Barry C. Starcher
    • 1
  1. 1.Pulmonary Division, Department of MedicineWashington University School of Medicine at The Jewish Hospital of St. LouisSt. LouisUSA

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