The Fate of Intratracheal 14C-Guanidinated Pancreatic Elastase in Hamster Lung
Native pancreatic elastase and guanidinated elastase have similar in vitro and in vivo properties and produce emphysema of similar severity in hamsters. 14C-guanidina-ted pancreatic elastase (16,000 cpm/0.2 mg) was instilled into the trachea of anesthetized hamsters. Within 24 hours the radiolabel found in the lungs rapidly decreases to 12% of the original 16,000 cpm and to 1% after 96 hours. Most of the radiolabel and elastase activity found in the lungs can be removed by bronchopulmonary lavage up to 48 hours after instillation. Although seemingly very small, there is a significant amount of radiolabel (1–2%) which cannot be removed from the lungs by extensive bronchopulmonary lavage.
KeywordsIntratracheal Instillation Elastase Activity Elastase Inhibitor Pancreatic Elastase Porcine Pancreatic Elastase
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- 1.Blackwood, C.E., Hosannah, Y., Perman, E., Keller, S. and Mandl, I. (1973) Proc. Soc. Exp. Biol. Med. 144, 450.Google Scholar
- 4.Shotton, D.M. in Methods in Enzymology, Vol. XIX. G.E. Perlman and L. Lorand, eds., Academic Press, New York, 1970, p. 113.Google Scholar
- 6.Kimmel, J.R. in Methods in Enzymology, Vol. XI, C.H.W. Hirs, ed., Academic Press, New York, 1967, p. 584.Google Scholar
- 10.Kovnat, D.M., Snider, G.L. and Brody, J.S. (1975) Clin. Res. 23, 349A.Google Scholar
- 13.Sandhaus, R.A. and Janoff, A. (1976) Amer. Rev. Resp. Dis. (Abstract) 113, 214.Google Scholar
- 15.Snider, G.L., Sherter, C.B., Koo, K.W., Karlinsky, J.B., Hayes, J.A. and Franzblau, C, J. Appl. Physiol., in press.Google Scholar