Proteolysis of Tropoelastin

  • Robert Mecham
  • Judith A. Foster
  • Carl Franzblau
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)


The presence of an enzyme associated with tropoelastin is described. The enzyme has a pH optimum between 7 and 9 and trypsin-like specificity. Upon incubation, tropoelastin (72,000 molecular weight) is cleaved into relatively high molecular weight fragments. In addition to the parent molecule, five discrete polypeptide bands are usually observed on SDS gels with molecular weights of approximately 57,000, 45,000, 36,000, 25,000 and 13–14,000.


Caproic Acid Soybean Trypsin Inhibitor Chloromethyl Ketone Lower Molecular Weight Band Diethyldithiocarbamic Acid 
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  1. 1.
    Byers, P.H., Click, E.M., Harper, E. and Bornstein, P., (1975) Proc. Natl. Acad. Sci 72, 3009.PubMedCrossRefGoogle Scholar
  2. 2.
    Fessier, L.I., Morris, N.P., and Fessier, J.H., (1975) Proc. Natl. Acad Sci 72, 4905.CrossRefGoogle Scholar
  3. 3.
    Foster, J.A., Mecham, R.P., and Franzblau, C, (1976) Biochem Biophys. Res. Comm., in press.Google Scholar
  4. 4.
    Foster, J.A., Shapiro, R., Voynow, P., Crombie, G., Faris, B., and Franzblau, C, (1975) Biochemistry 14, 5343.PubMedCrossRefGoogle Scholar
  5. 5.
    Mecham, R.P., Foster, J.A., Franzblau, C, (1976) Biochim. Biophys Acta, in press.Google Scholar
  6. 6.
    Mecham, R.P., Foster, J.A., Franzblau, C, (1976) in preparation.Google Scholar
  7. 7.
    Robert, L., Moczar, M., and Robert, M., (1974) Experientia 30, 211.PubMedCrossRefGoogle Scholar
  8. 8.
    Sandberg, L.B., Bruenger, E., and Cleary, E.G., (1975) Anal. Biochem. 64, 249.PubMedCrossRefGoogle Scholar
  9. 9.
    Sandberg, L.B., Weissman, N., and Smith, D.W. (1969) Biochemistry 8, 2940.PubMedCrossRefGoogle Scholar
  10. 10.
    Smith, D.W., Brown, D., Carnes, W.H., (1972) J. Biol. Chem. 247, 2427.PubMedGoogle Scholar
  11. 11.
    Sykes, B.C., and Partridge, S.M., (1972) Biochem. J. 130, 1171.PubMedGoogle Scholar
  12. 12.
    Sykes, B.C., and Partridge, S.M., (1974) Biochem. J. 141, 567.PubMedGoogle Scholar
  13. 13.
    Weissman, N., Shields, G.S., and Carnes, W.H., (1963) J. Biol. Chem. 238, 3115.PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • Robert Mecham
    • 1
  • Judith A. Foster
    • 1
  • Carl Franzblau
    • 1
  1. 1.Department of BiochemistryBoston University School of MedicineBostonUSA

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