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Photolysis of Desmosine And Isodesmosine by Ultraviolet Light

  • F. Lamy
  • R. Bauraln
  • M. Guay
  • J. F. Larochelle
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)

Abstract

It is known that the pyridinium ring of a model compound such as N-methyl pyridinium chloride is cleaved by U.V. radiation at 254 nm. At acid pH the products obtained are methylamine and glutaconaldehyde. Under the same conditions desmosine and isodesmosine are degraded into lysine and probably into the homologous substituted ketone of glutaconaldehyde. At pH 6.0–7.0, a transient open-chain aminoaldehyde intermediate is observed which can either reform the original compound or be cleaved as at low pH. When intact elastin is photolysed for 20 minutes in water, approximately 75% of the (iso) desmosines are destroyed, accompanied by an increase of free lysine residues. No change in the concentration of the other amino acids, including tyrosine, are noted. It is therefore likely that the crosslinks engaged in peptide links are also cleaved photochemically.

Keywords

Peptide Bond Amino Acid Analysis Pyridinium Ring Order Rate Constant Soluble Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • F. Lamy
    • 1
  • R. Bauraln
    • 1
  • M. Guay
    • 1
  • J. F. Larochelle
    • 1
  1. 1.Departement de Biochimie, Faculte de MedecineUniversite de SherbrookeSherbrookeCanada

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