Advertisement

Recent Observations on the Structure and Composition of Elastin

  • L. Gotte
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 79)

Abstract

The ultrastructural organization of elastin has been studied by scanning and transmission electron microscopy of negatively stained specimens coupled with optical diffraction analyses.

Purified elastin results from a composite assembly of interwoven branched and twisted thread-like entities of decreasing diameter. The ultimate filamentous component is a rope-like structure each with an overall diameter of 3.5–4.0 nm consisting of paired filaments of indefinite length each 1.5 nm in width and periodically linked longitudinally at a varying distance of 3.5 to 4.5 nm depending on the degree of stretch.

Keywords

Elastic Fiber Optical Diffraction Elastin Fibre Intercellular Matrix Paired Filament 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cleary, E.G., Jackson, D.S. and Sandberg, L.B., Changes in Amino Acid Composition of Bovine Ligamentum Elastin with Ageing. Biochimie et Physiologie du Tissue Conjonctif in Comte P., (Ed), p. 167. Lyon 1966.Google Scholar
  2. 2.
    Gotte, L., Serafini-Fracassini, A. and Moret, V., The Chemical Composition of the NaCl Soluble Fraction from Autoclaved Elastin. J. Atheroscler. Res. 3, 244 (1963).PubMedCrossRefGoogle Scholar
  3. 3.
    GreenTee, T.K., Ross, R. and Hartman J.L., The Fine Structure of Elastic Fibers. J. Cell Biol. 30, 59 (1966).CrossRefGoogle Scholar
  4. 4.
    Ross, R. and Bornstein, P., The Elastic Fiber. I The Separation and Partial Characterization of Its Macromol ecul ar Components. J. Cell Biol. 40, 366 (1969).PubMedCrossRefGoogle Scholar
  5. 5.
    Moret, V. Gotte, L., and Serafini-Fracassini, A., The Carbohydrate Composition of NaCl Soluble Fraction from Autoclaved Elastin. J. Atheroscler. Res. 4, 184 (1964).PubMedCrossRefGoogle Scholar
  6. 6.
    Weis-Fogh T., Thermodynamic Properties of Resilin, a Rubberlike Protein. J. Mol. Biol. 3, 520 (1961).CrossRefGoogle Scholar
  7. 7.
    Thornhill, D.P., Abductin-Locus and Spectral Characteristics of a Brown, Fluorescent Chromophore. Biochemistry 13, 2644 (1971).CrossRefGoogle Scholar
  8. 8.
    Lansing, A.T., Rosenthal, T.B., Alex, M. and Dempsey, E.W., The Structure and Characterization of Elastic Fibers as Revealed by Elastase and by Electron Microscopy. Am. Rec. 114, 555 (1952).CrossRefGoogle Scholar
  9. 9.
    Partridge, S.M., Elastin Structure and Biosynthesis. “Symposium on Fibrous Proteins” Crewther W.G., Ed. Butterworths, Australia pp. 246–264 (1968).Google Scholar
  10. 10.
    Ross, R. and Bornstein P., Studies of the Components of the Elastic Fiber, in “Chemistry and Molecular Biology of the Intercellular Matrix” (Edit, by Balazs) I, 641, Academic Press, London 1970.Google Scholar
  11. 11.
    Serafini-Fracassini, A., Field, M., Rodger, G.W. and Spina, M., Application of Affinity Chromatography to the Purification of Collagenase for the Isolation of Insoluble Elastin. Biochim. et Biophys. Acta 386, 80 (1975).CrossRefGoogle Scholar
  12. 12.
    Gotte, L., Stern, P., Elsden, D.F. and Partridge S.M., The Chemistry of Connective Tissue. The Composition of Elastin from Three Bovine Tissues. Biochem. J. 87, 344 (1963).PubMedGoogle Scholar
  13. 13.
    Kagan H.M. and Lerch, R.M., Amidated Carboxyl Groups in Elastin. Biochim. et Biophys. Acta 434, 223 (1976).CrossRefGoogle Scholar
  14. 14.
    Thomas, J., Elsden, D.F. and Partridge, S.M., Partial Structure of the Two Major Degradation Products from the Cross”! inkages in elastin. Nature 200, 651 (1963).PubMedCrossRefGoogle Scholar
  15. 15.
    Partridge, S.M., Isolation and Characterization of Elastin. in “Chemistry and Molecular Biology of the Intercellular Matrix” (edit, by Balazs E.A.) I, 593 Academic Press, London 1970.Google Scholar
  16. 16.
    Hoeve, C.A.J, and Flory, P.J., The Elastic Properties of Elastin. Bioploymers, 13, 677 (1974).CrossRefGoogle Scholar
  17. 17.
    Volpin, D. and Ciferri, A., The Elastic Behaviour of Elastin. in “Chemistry and Molecular Biology of the Intercellular Matrix” (Ed. by Balazs E.A.) I, 691, Academic Press, London 1970.Google Scholar
  18. 18.
    Mistrali, F., Volpin, D., Garibaldo, B.B. and Ciferri, A. Thermodynamics of Elasticity in Open Systems. Elastin. J. Phys. Chem. 75, 142 (1971).CrossRefGoogle Scholar
  19. 19.
    Weis-Fogh, T. and Andersen, S.O., Elasticity and thermodynamics of elastin. in “Chemistry and Molecular Biology of the Intercellular Matrix” (edit. by Balazs, E.A.) I, 671, Academic Press, London 1970.Google Scholar
  20. 20.
    Dorrington, K., Crut, W. and McCrum, N.G., Mechanical state of elastin. Nature 225, 476 (1975).CrossRefGoogle Scholar
  21. 21.
    Gotte, L., Mammi, M., Pezzin, G., Scanning electron microscope observation on elastin. Connect. Tissue Res. I, 61 (1972).CrossRefGoogle Scholar
  22. 22.
    Gotte, L, Giro, M.G., Volpin, D. and Horne, R.W., The ultrastructural organisation of elastin. J. Ultrastruct. Res. 46, 23 (1974).PubMedCrossRefGoogle Scholar
  23. 23.
    Gotte, L., Volpin, D., Home, R.W. and Mammi, M., Electron microscopy and optical diffraction of elastin. Micron, in press (1976).Google Scholar

Copyright information

© Plenum Press, New York 1977

Authors and Affiliations

  • L. Gotte
    • 1
  1. 1.Institute of HistologyUniversity of PaduaPaduaItaly

Personalised recommendations