Biomembranes pp 131-165 | Cite as

Organization of Glycoprotein and Glycolipid in the Plasma Membrane of Normal and Transformed Cells as Revealed by Galactose Oxidase

  • Carl G. Gahmberg
  • Sen-itiroh Hakomori
Part of the Biomembranes book series (B, volume 8)


Plasma membranes of mammalian cells have been characterized by their higher content of glycosphingolipids (Weinstein et al., 1967; Dod and Gray, 1968; Klenk and Choppin, 1970; Renkonen et al., 1970; Yogeeswaran et al., 1972; Critchley et al., 1973) and by the presence of a particular glycoprotein, as indicated by the higher incorporation of radioactive fucose or glucosamine into isolated plasma membranes (Gahmberg, 1971). In fact, the amounts of glycolipids and of protein-bound fucose are good markers for plasma membranes (Renkonen et al., 1970; Gahmberg, 1971). During the last several years, surface carbohydrates have been implicated in a variety of biological phenomena, such as cellular adhesion (Roseman, 1971; Roth and White, 1972), the lymphocyte homing phenomenon (Gesner and Ginsburg, 1964), lectin-induced agglutination of transformed cells (Burger, 1969; Inbar and Sachs, 1969), specific recognition of homologous cells by tectal cells during a particular term of development (Gottlieb et al., 1974), and histotypic aggregation of retinal cells (Lilien and Moscona, 1967; Moscona, 1971) and of sponge cells (Humphrey, 1963).


Sponge Cell Polyoma Virus Human Erythrocyte Membrane Surface Label Galactose Oxidase 
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Copyright information

© Plenum Press, New York 1976

Authors and Affiliations

  • Carl G. Gahmberg
    • 1
  • Sen-itiroh Hakomori
    • 2
  1. 1.Departments of Pathobiology and MicrobiologyUniversity of WashingtonSeattleUSA
  2. 2.The Fred Hutchinson Cancer Research CenterSeattleUSA

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