Organization of Glycoprotein and Glycolipid in the Plasma Membrane of Normal and Transformed Cells as Revealed by Galactose Oxidase
Plasma membranes of mammalian cells have been characterized by their higher content of glycosphingolipids (Weinstein et al., 1967; Dod and Gray, 1968; Klenk and Choppin, 1970; Renkonen et al., 1970; Yogeeswaran et al., 1972; Critchley et al., 1973) and by the presence of a particular glycoprotein, as indicated by the higher incorporation of radioactive fucose or glucosamine into isolated plasma membranes (Gahmberg, 1971). In fact, the amounts of glycolipids and of protein-bound fucose are good markers for plasma membranes (Renkonen et al., 1970; Gahmberg, 1971). During the last several years, surface carbohydrates have been implicated in a variety of biological phenomena, such as cellular adhesion (Roseman, 1971; Roth and White, 1972), the lymphocyte homing phenomenon (Gesner and Ginsburg, 1964), lectin-induced agglutination of transformed cells (Burger, 1969; Inbar and Sachs, 1969), specific recognition of homologous cells by tectal cells during a particular term of development (Gottlieb et al., 1974), and histotypic aggregation of retinal cells (Lilien and Moscona, 1967; Moscona, 1971) and of sponge cells (Humphrey, 1963).
KeywordsSponge Cell Polyoma Virus Human Erythrocyte Membrane Surface Label Galactose Oxidase
Unable to display preview. Download preview PDF.
- Avigad, G., Amaral, D., Asensio, C., and Horecker, B. L., 1962, The D-galactose oxidase of Polyporus circinatus, J. Biol. Chem. 237:2736.Google Scholar
- Cooper, J. A. D., Smith, W., Bacila, M., and Medina, H., 1959, Galactose oxidase of Polyporus circinatus, J. Biol. Chem. 234:445.Google Scholar
- Critchley, D. R., and Macpherson, I., 1973, Cell density-dependent glycolipids in NIL2 hamster cells derived from malignant and transformed cell lines, Biochim. Biophys. Acta 246:145.Google Scholar
- Gahmberg, C. G., and Hakomori, S., 1975a, Surface carbohydrates of hamster fibroblasts I. Chemical characterization of surface-labeled glycosphingolipids and a specific ceramide tetrasaccharide for transformants, J. Biol. Chem. 250:2438.Google Scholar
- Hakomori, S., 1973, Glycolipids of tumor cell membranes, in: Advances in Cancer Research, Vol. 18 (S. Weinhouse, ed.), pp. 265–315, Academic Press, New York.Google Scholar
- Hakomori, S., and Kobata, A., 1975, Blood group antigens, in: The Antigens, Vol. 2 (M. Sela, ed.), Academic Press, New York.Google Scholar
- Laine, R. A., Esselman, W. J., and Sweeley, C. C., 1972, Gas liquid chromatography of carbohydrates, in: Methods of Enzymology, Vol. 28 (V. Ginsburg, ed.), pp. 159–167, Academic Press, New York.Google Scholar
- Liao, T. H., Gallop, P. M., and Blumenfeld, O., 1973, Modification of sialyl residues of sialoglycoprotein(s) of the human erythrocyte surface, J. Biol. Chem. 218:8247.Google Scholar
- Rice, R. H., and Means, G. E., 1971, Radioactive labeling of proteins in vitro, J. Biol. Chem. 246:831.Google Scholar
- Singer, S. J., 1974, The molecular organization of membranes, in: Annual Review of Biochemistry (F. Fox, ed.), p. 866, Annual Reviews, Inc., Palo Alto, Calif.Google Scholar
- Steck, T. L., 1972, The organization of proteins in human erythrocyte membranes, in: Membrane Research (C. Fox, ed.), pp. 71–93, Academic Press, New York.Google Scholar
- Weinstein, D. B., Warren, L., Marsh, J. B., and Glick, M. C., 1967, Glycolipids of L-cell membrane, Fed. Proc. 28:3654.Google Scholar