Biochemical Determinants in the Evaluation of Monoamine-Oxidase Inhibitors

  • Sabit Gabay
  • Alfred J. Valcourt


This paper will undertake a critical discussion of recently observed interactions of certain antidepressants with highly purified and partially characterized liver mitochondrial monoamine-oxidase (MAO) preparations (rabbit and pig) and their possible mechanisms of action.


Monoamine Oxidase Monoamine Oxidase Inhibitor Chelate Agent Federation Proc Biochemical Evaluation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Gabay, S., Valcourt, A., and MacKeen, D.: Preparation of highly-purified liver monoamine oxidase. Federation Proc. 25:319, 1966.Google Scholar
  2. 2.
    Gabay, S., Valcourt, A., and Valanju, N.: On the inhibition mechanism of a highly-purified liver monoamine oxidase (MAO), The Pharmacologist 8:196, 1966.Google Scholar
  3. 3.
    Valcourt, A., Valanju, N., and Gabay, S.: On the inhibition mechanism of antidepressants. Federation Proc. 26:652, 1967.Google Scholar
  4. 4.
    Gabay, S., and Valcourt, A. J.: Studies of monoamine oxidases. I. Purification and properties of the rabbit liver mitochondrial enzyme (in press).Google Scholar
  5. 5.
    Weissbach, H., Smith, T.E., Daly, J.W., Witkop, B., and Udenfriend, S.: A rapid spectro-photometric assay of monoamine oxidase based on the rate of disappearance of kynuramine, J. Biol. Chem. 235:1160, 1960.PubMedGoogle Scholar
  6. 6.
    Weikel, J.H., Jr., and Salmon, J.E.: Biochemical properties of N-benzyl-N-isopropyl-hydrazine, Biochem. Pharmacol. 11:93, 1962.Google Scholar
  7. 7.
    Barbato, L.M., and Abood, L.G.: Purification and properties of monoamine oxidase, Biochim. Biophys. Acta 67:531, 1963.CrossRefGoogle Scholar
  8. 8.
    Zeller, V., Ramachander, G., and Zeller, E. A.: Amine oxidases. XXI. A rapid method for the determination of the activity of monoamine oxidase and monoamine oxidase inhibitors, J. Med. Chem. 8:440, 1965.PubMedCrossRefGoogle Scholar
  9. 9.
    Zeller, E.A.: Monoamine and polyamine analogues, in: Hochster, R.M., and Quastel, J.H. (eds.), Metabolic Inhibitors, Vol. 2, Academic Press, New York, 1963, p. 68.Google Scholar
  10. 10.
    Valcourt, A.J., and Gabay, S.: The effects of antidepressants on highly-purified rabbit liver monoamine oxidase (in preparation).Google Scholar
  11. 11.
    Biel, J.H., Horita, A., and Drukker, A.E.: Monoamine oxidase inhibitors (hydrazines), in: Gordon, M. (ed.), Psychopharmacological Agents, Vol. 1, Academic Press, New York, 1964, p. 422.Google Scholar
  12. 12.
    Zirkle, C.L., Kaiser, C., Tedeschi, D.H., Tedeschi, R.E., and Burger, A.: 2-Substituted cyclopropylamines. II. Effect of structure upon monoamine oxidase inhibitory activity, J. Med. Pharm. Chem. 5:1265, 1962.CrossRefGoogle Scholar
  13. 13.
    Tedeschi, D.H., Tedeschi, R.E., and Fellows, E.J.: In vivo monoamine oxidase inhibition measured by potentiation of tryptamine convulsions in rats, Proc. Soc. Exper. Biol. Med. 103:680, 1960.Google Scholar
  14. 14.
    Pletscher, A., Gey, K.F., and Zeller, P.: Monoaminoxydase-Hemer: Chemie, Biochemie, Pharmacologie, Klinik, Progr. Drug Res. 2:417, 1960.Google Scholar
  15. 15.
    Brodie, B.B., Spector, S., and Shore, P.A.: Interaction of monoamine oxidase inhibitors with physiological and biochemical mechanisms in brain, Ann. N.Y. Acad. Sci. 80:609, 1959.PubMedCrossRefGoogle Scholar
  16. 16.
    Schildkraut, J.J., and Kety, S.S.: Biogenic amines and emotion, Science 156:21, 1967.PubMedCrossRefGoogle Scholar
  17. 17.
    Häfliger, F., and Burckhardt, V.: Iminodibenzyl and related compounds, in: Gordon, M. (ed.), Psychopharmacological Agents, Vol. 1, Academic Press, New York, 1964, p. 77.Google Scholar
  18. 18.
    Pletscher, A., and Gey, K.F.: Pharmakologische Beeinslussung des 5-Hydroxytryptamine-Stoffwechseis im Gehirn und Monoaminoxydasehemmung fnVitro, Helv. Physiol. Pharmacol. Acta 17:C35. 1959.Google Scholar
  19. 19.
    Himwich, W.A., and Petersen, J.C.: Interaction of imipramine with monoamine oxidase inhibitors. Federation Proc. 20:394, 1961.Google Scholar
  20. 20.
    Kivalo, E., Rinne, U.K., and Karinkata, H.: The effect of imipramine on the 5-hydroxy-tryptamine content and monoamine oxidase activity of the rat brain, J. Neurochem. 8:105, 1961.PubMedCrossRefGoogle Scholar
  21. 21.
    McEwen, C.M., Jr.: Human plasma monoamine oxidase. I. Purification and identification, J. Biol. Chem. 240:2003, 1965.Google Scholar
  22. 22.
    Vitek, V., and Rysanek, D.: Interaction of D-cycloserine with the action of some monoamine oxidase inhibitors, Biochem. Pharmacol. 14:1417, 1965.PubMedCrossRefGoogle Scholar
  23. 23.
    Gogerty, J.H., and Horita, A.: A comparison of the in vivo inhibition of brain and liver monoamine oxidase as produced by beta-phenylisopropylhydrazine (PIH) and iproniazid, J. Pharmacol. Exp. Therap. 129:357, 1960.Google Scholar
  24. 24.
    Diehl, H., and Smith, G.F.: The Copper Reagents, G. Frederick Smith Chemical Co., Columbus, Ohio, 1958.Google Scholar
  25. 25.
    Frieden, E.: The Complex Copper of Nature, in: Kasha, M., and Pullman, B. (eds.). Horizons in Biochemistry, Academic Press, New York, 1962, p. 461.Google Scholar
  26. 26.
    Stark, G.R., and Dawson, C.R.: Ascorbic acid oxldase, in: Boyer, P. D., Lardy, H., and Myrbäck, K. (eds.), Enzymes, Vol. VIII, Academic Press, New York, 1963, p. 297.Google Scholar
  27. 27.
    Nara, S., Gomes, B., and Yasunobu, K.T.: Amine oxidase. VII. Beef liver mitochondrial monoamine oxidase, a copper containing protein, J. Biol. Chem. 241:2774, 1966.PubMedGoogle Scholar
  28. 28.
    Guha, S.R.: Inhibition of purified rat liver mitochondrial monoamine oxidase by trans-2— phenylcyclopropylamine, Biochem. Pharmacol. 15:161, 1966.PubMedCrossRefGoogle Scholar
  29. 29.
    Erwin, V.G., and Hellerman, L.: Mitochondrial monoamine oxidase. Characterization of the bovine kidney enzyme. Federation Proc. 26:843, 1967.Google Scholar
  30. 30.
    Zile, M., and Lardy, H.A.: Monoamine oxidase activity in liver of thyroid-fed rats. Arch. Biochem. Biophys. 82:411, 1959.PubMedCrossRefGoogle Scholar
  31. 31.
    Novick, W.J., Jr.: The effect of age and thyroid hormones on the monoamine oxidase of rat heart. Endocrinology 69:55, 1961.PubMedCrossRefGoogle Scholar
  32. 32.
    Peisach, J., Aisen, P., and Blumberg, W.E.: The Biochemistry of Copper, Academic Press, New York, 1966.Google Scholar
  33. 33.
    Curzon, G.: The effects of some ions and chelating agents on the oxidase activity of caerulo-plasmin, Biochem. J. 77:66, 1960.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1968

Authors and Affiliations

  • Sabit Gabay
  • Alfred J. Valcourt

There are no affiliations available

Personalised recommendations