Immunoregulatory Factors Produced by Activated Lymph Nodes In Vivo

  • Leonard S. English
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 149)


The induction of antigen-specific lymphocytes by antigen, their subsequent proliferation and production of an effector function is, to a large extent, regulated by a family of molecules that may act to specifically or nonspecifically augment or suppress the immune response, A major proportion of the work on the production of these factors has, of necessity, been performed in vitro, and we are deficient in the knowledge of how and when they are produced in vivo. The collection of immune cells from the efferent lymphatics of locally-stimulated lymph nodes in sheep1 provides a possible in vivo model to correct some of these deficiencies. Using this model, I have demonstrated that efferent cells collected from lymph during the first few hours of responses to dinitrophenylated bovine serum albumin (DNP-BSA) and Concanavalin A (Con A) exhibited up to 7-fold enhanced responses to low doses of phytohemagglutinin (PHA) in vitro 2,3. Other workers have reported a nondialyzable mitogenic factor in immune lymph collected at a similar time during responses to Con A4 and tuberculin5, but no further characterization of the responsible factors have been forthcoming. In this paper I describe the isolation and partial characterization of synergizing/mitogenic factors found in lymph during the first 12hr of the response to a variety of antigens.


Migration Inhibition Factor Activate Lymph Node Mitogenic Activity Popliteal Node Allogeneic Cell 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Leonard S. English
    • 1
  1. 1.Department of MicrobiologyEast Carolina University School of MedicineGreenvilleUSA

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