Distribution and Functional Properties of PNA+ and PNA- Cells in Central and Peripheral Lymphoid Organs of the Chicken
Peanut agglutinin (PNA) has turned out to be a potent tool to distinguish between cortical and medullary lymphocytes in the mouse thymus (1,2). In addition, more recent studies revealed that PNA may preferentially bind to cells acting as suppressors. PNA+ suppressor cells were detected in the fetal liver (3) and aging mouse spleen (4), as well as in the thymus of young adult animals (5). Furthermore, antigen-specific suppressors in the adult spleen were also characterized as PNA+ (6,7). Whereas the thymic and the antigen-specific splenic suppressor cells have been identified as T cells, the embryonic ones, and those appearing in aging were not eliminated by anti Thy 1.2 antibodies and complement (7 and unpublished observations). Hence, PNA seems to bind to suppressor cells of various membrane phenotypes. The possibility that PNA may enable identification and separation of various types of suppressor cells within the different lymphoid cell compartments thus seemed intriguing.
KeywordsSpleen Cell Suppressor Cell Mixed Lymphocyte Reaction Chicken Serum Peanut Agglutinin
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