Characterization of Bone Marrow Derived Macrophage as Ia-Bearing Accessory Cells

  • Lawrence B. Schook
  • David H. Gutmann
  • John E. Niederhuber
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 149)


Genetic restrictions operative in macrophage-T cell interactions have been shown to reside in the I region of the mouse H-2 major histocompatibility complex (1,2). Previous experiments to determine the role of Ia antigens in macrophage-T cell interactions have used macrophage isolated from spleen or peritoneum, preparations which contain a heterogeneous population of macrophage (3–5). It has been shown that only macrophage populations which express Ia are capable of presenting antigens to immune cells (4,5). Therefore, the use of a macrophage preparation homogeneously expressing I subregion antigens would afford a valuable opportunity to examine the role of Ia antigens in the regulation of the immune response. The use of murine bone marrow derived macrophage (BMDM) as a homogeneous source of antigen-presenting cells has been described by our laboratory (6). This present study characterized the MBDM for the expression of H-2I region gene products.


Mixed Lymphocyte Reaction Bone Marrow Derive Macrophage Murine Bone Marrow Splenic Macrophage Homogeneous Source 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Lawrence B. Schook
    • 1
  • David H. Gutmann
    • 1
  • John E. Niederhuber
    • 1
  1. 1.Department of Microbiology and Immunology, Department of SurgeryUniversity of Michigan Medical SchoolAnn ArborUSA

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