Characterization of Bone Marrow Derived Macrophage as Ia-Bearing Accessory Cells
Genetic restrictions operative in macrophage-T cell interactions have been shown to reside in the I region of the mouse H-2 major histocompatibility complex (1,2). Previous experiments to determine the role of Ia antigens in macrophage-T cell interactions have used macrophage isolated from spleen or peritoneum, preparations which contain a heterogeneous population of macrophage (3–5). It has been shown that only macrophage populations which express Ia are capable of presenting antigens to immune cells (4,5). Therefore, the use of a macrophage preparation homogeneously expressing I subregion antigens would afford a valuable opportunity to examine the role of Ia antigens in the regulation of the immune response. The use of murine bone marrow derived macrophage (BMDM) as a homogeneous source of antigen-presenting cells has been described by our laboratory (6). This present study characterized the MBDM for the expression of H-2I region gene products.
KeywordsMixed Lymphocyte Reaction Bone Marrow Derive Macrophage Murine Bone Marrow Splenic Macrophage Homogeneous Source
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