Advertisement

Monoclonal Antibodies to Cell Surface Antigens on Lymphoid and Non-Lymphoid Cells of the Mouse

  • P. W. van Vliet
  • W. van Ewijk
  • W. A. M. Boere
  • P. L. van Soest
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 149)

Abstract

The structural basis of the complex micro-architecture of lymphoid microenvironments is still poorly understood. Further insight into the structure and function of various lymphoid microenvironments, and their role in the differentiation of subpopulations of lymphocytes can be obtained when the distribution of cell-type specific surface markers is included in histological studies. We have previously employed monoclonal antibodies directed against H-2 antigens, Thy-1, T-200 and Lyt antigens to analyze lymphoid microenvironments in frozen sections of the thymus and peripheral lymphoid organs (Rouse et al., 1979; Van Ewijk et al., 1980a, 1980b, 1981). In the present paper we describe the reactivities of five monoclonal antibodies, recently produced in our own laboratory to mouse (C3H) thymocytes (Van Vliet et al., 1980) and we compare these reactivities with well defined monoclonal anti-Thy-1, anti-Lyt-1 and anti-Lyt-2 antisera. To this purpose we have used an ELISA developed by Lansdorp et al. (1980), immunohistology and flow cytofluorometric analysis.

Keywords

Spleen Cell Marginal Zone Cell Surface Antigen Negative Control Cell Fluorescence Profile 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    W. van Ewijk, R.C. Coffman, and I.L. Weissman. Histochem. J. 12:349 (1980a).PubMedCrossRefGoogle Scholar
  2. 2.
    W. van Ewijk, R.V. Rouse, and I.L. Weissman. J. Histochem. Cytochem. 28:1089 (1980b).PubMedCrossRefGoogle Scholar
  3. 3.
    W. van Ewijk, P.L. van Soest, and G.J. van der Engh. Submitted for publication.Google Scholar
  4. 4.
    P.M. Lansdorp, G.C.B. Astaldi, F. Oosterhof, M.C. Janssen, W.P. Zeijlemaker. J. Immunol. Meth. 39:393 (1980).CrossRefGoogle Scholar
  5. 5.
    J.A. Ledbetter, and L.A. Herzenberg. Immunol. Rev. 47:63 (1979).PubMedCrossRefGoogle Scholar
  6. 6.
    M.R. Loken, and L.A. Herzenberg. Ann. N.Y. Acad. Sci. 254: 163 (1975).PubMedCrossRefGoogle Scholar
  7. 7.
    R.V. Rouse, W. van Ewijk, P.P. Jones, and I.L. Weissman. J. Immunol. 122:2508 (1979).PubMedGoogle Scholar
  8. 8.
    P.W. van Vliet, W. van Ewijk, J. Rozing, and P.L. van Soest in: “Protides of the Biological Fluids”, 28th Colloquium, p. 553–557, H. Peeters, ed., Pergamon Press, Oxford (1980).Google Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • P. W. van Vliet
    • 1
  • W. van Ewijk
    • 1
  • W. A. M. Boere
    • 1
  • P. L. van Soest
    • 1
  1. 1.Dept. Cell Biology & GeneticsErasmus UniversityDR RotterdamThe Netherlands

Personalised recommendations