Frequency and Restriction of Cytotoxic T Lymphocyte Precursors in Neonatally Thymectomized Mice

  • Claude Carnaud
  • Tehilah Umiel
  • Louis Gastinel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 149)


Neonatal thymectomy causes a severe and definitive impairment of all T-cell functions (1). This fact has been considered for a long time as an indication that T-lymphocytes migrate obligatorily through the thymus, and that competent effector cells appear in the peripheral organs following a delay after birth. However, we have recently shown the presence, in the spleen of neonatally thymectomized (N-Tx) mice, of competent cytotoxic T lymphocyte precursors (CTL-P) (2). These CTL-P can be specifically activated in vitro upon antigenic stimulation in the presence of supernatants from Con A activated cells (Con A sup) or of a thymic humoral factor (THF). In this report, we provide further information about this pool of CTL-P. We have estimated their frequency and their degree of H-2 restriction in comparison with CTL-P which have differentiated under normal thymic influence.


Spleen Cell Peripheral Organ Maximal Likelihood Analysis Intact Mouse Responder Cell 
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Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Claude Carnaud
    • 1
  • Tehilah Umiel
    • 2
  • Louis Gastinel
    • 1
  1. 1.INSERM U 25Hopital NeckerParis Cedex 15France
  2. 2.Department of Cell BiologyWeizman Institute of SciencesRehovotIsrael

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