Frequency and Restriction of Cytotoxic T Lymphocyte Precursors in Neonatally Thymectomized Mice

  • Claude Carnaud
  • Tehilah Umiel
  • Louis Gastinel
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 149)


Neonatal thymectomy causes a severe and definitive impairment of all T-cell functions (1). This fact has been considered for a long time as an indication that T-lymphocytes migrate obligatorily through the thymus, and that competent effector cells appear in the peripheral organs following a delay after birth. However, we have recently shown the presence, in the spleen of neonatally thymectomized (N-Tx) mice, of competent cytotoxic T lymphocyte precursors (CTL-P) (2). These CTL-P can be specifically activated in vitro upon antigenic stimulation in the presence of supernatants from Con A activated cells (Con A sup) or of a thymic humoral factor (THF). In this report, we provide further information about this pool of CTL-P. We have estimated their frequency and their degree of H-2 restriction in comparison with CTL-P which have differentiated under normal thymic influence.


Spleen Cell Peripheral Organ Maximal Likelihood Analysis Intact Mouse Responder Cell 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    J.F.A.P. Miller, and G.F. Mitchell. Transplant. Rev. 1:3 (1969).PubMedGoogle Scholar
  2. 2.
    T. Umiel, B. Klein, W. Droge, and N. Trainin. Submitted for publication.Google Scholar
  3. 3.
    I. Lefkovits, and H. Waldmann. Cambridge University Press, Cambridge (1979).Google Scholar
  4. 4.
    P.C. Doherty, R.V. Blanden, and R.M. Zinkernagel. Transpl. Rev. 89:29 (1976).Google Scholar
  5. 5.
    S.J. Burakoff, R.N. Germain, and B. Benacerraf. J. Exp. Med. 144:1609 (1976).PubMedCrossRefGoogle Scholar
  6. 6.
    R.F. Zinkernagel, G. Callahan, J. Klein, and G. Dennert. Nature 271:251 (1978).PubMedCrossRefGoogle Scholar
  7. 7.
    O. Stutman. Immunol. Rev. 42:138 (1978).PubMedCrossRefGoogle Scholar
  8. 8.
    S. Gillis, N.A. Union, P.E. Baker, and K.A. Smith. J. Exp. Med. 149:1460 (1979).PubMedCrossRefGoogle Scholar
  9. 9.
    J.L. Maryanski, H.R. MacDonald, B. Sordat, and J.C. Cerottini. J. Immunol. 126:871 (1981).PubMedGoogle Scholar
  10. 10.
    T. Hunig, and M. Bevan. J. Exp. Med. 152:688 (1980).PubMedCrossRefGoogle Scholar
  11. 11.
    R. Bartlett, K. Pfizenmaier, C. Hardt, M. Rollinghoff, and H. Wagner. Behring Inst. Res. Communications 67:123 (1980).Google Scholar
  12. 12.
    W. Droge. Cell. Immunol. 57:251 (1981).PubMedCrossRefGoogle Scholar
  13. 13.
    S.J. Burakoff, J. Riccio, P. Billings et al. J. Immunol. 125:1432 (1980).PubMedGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • Claude Carnaud
    • 1
  • Tehilah Umiel
    • 2
  • Louis Gastinel
    • 1
  1. 1.INSERM U 25Hopital NeckerParis Cedex 15France
  2. 2.Department of Cell BiologyWeizman Institute of SciencesRehovotIsrael

Personalised recommendations