Unilateral Localization of Hapten-Specific B Memory Cells in Lymph Node Draining a Footpad Injection of Antigen
In previous studies, rabbits were found to exhibit higher immunological memory responses in lymph nodes draining a footpad injection of antigen than in lymph nodes from the contralateral side1. This difference was demonstrated by the magnitude and quality of responses in vitro and in vivo at various intervals after a single priming injection1–3. In unpublished observations in our laboratory a difference between lymph nodes from the left (L) and right (R) sides could still be observed 2 years after priming. Several authors3,4 have also observed that draining lymph nodes (LNs) may give “spontaneous” antibody responses even without a second challenge with antigen in vitro. This was suggested to be due to persisting antigen, particularly since added antibody could block this response5. Recent studies in mice have re-emphasized a role for persisting antigen in mediating such a prolonged ability of lymph node cells to give responses in vitro or upon transfer without antigen challenge6.
KeywordsMemory Cell Spleen Cell Drain Lymph Node Antibody Synthesis Left Lymph Node
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