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Fluorescence Activated Cell Sorter Analysis of Murine B Cell Development

  • J. J. Haaijman
  • W. J. A. Boersma
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 149)

Summary

Three subpopulations of surface immunoglobulin positive B cells were defined using monoclonal antibodies directed against IgM and IgD antigenic determinants and 2-color fluorescence activated cell sorter analysis: cells with a high density of sIgM and lacking sIgD (M++D-); cells with slightly less sIgM and dully positive for sIgD (M++D+), and cells with a low density of sIgM but brightly staining with anti-IgD (M+D+). In the spleen of two-week-old animals the M++D- cells are the more numerous. Their frequency declines during development in favor of the adult M+D+ cells. Because the densities of sIgM and sIgD on M+D+ cells are positively correlated it is hypothesized that immature M++D- cells first lose their sIgM and then reaquire sIgM together with sIgD.

Keywords

Developmental Type Spleen Cell Adult Bone Marrow Fluorescence Activate Cell Sorter Analysis Surface Immunoglobulin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. E.A. Bayer, and M. Wilchek. Methods in Enzymol. 34:265 (1974).CrossRefGoogle Scholar
  2. J.C. Cambier, J.R. Kettman, E.S. Vitetta, and J.W. Uhr. J. Exp. Med. 144:293 (1976).PubMedCrossRefGoogle Scholar
  3. J.W. Goding, D.W. Scott, and J.E. Layton. Immunol. Rev. 37:152 (1977).PubMedCrossRefGoogle Scholar
  4. J.J. Haaijman, in: “Advanced Immunofluorescence”, G. Wick, ed. (1981) in press.Google Scholar
  5. J.J. Haaijman, H.S. Micklem, J.A. Ledbetter, J.L. Dangl, L.A. Herzenberg, and L.A. Herzenberg. J. Exp. Med. 153:605 (1981).PubMedCrossRefGoogle Scholar
  6. L.A. Herzenberg, and L.A. Herzenberg, in: “Handbook of Experimental Immunology”, 3rd edition, chapter 22, D.M. Weir, ed. Blackwell Scientific Publications, Oxford (1978).Google Scholar
  7. J.A. Ledbetter, and L.A. Herzenberg. Immunol. Rev. 47:63 (1979).PubMedCrossRefGoogle Scholar
  8. M.R. Loken, D.R. Parks, and L.A. Herzenberg. J. Histochem. Cytochem. 25:899 (1977).PubMedCrossRefGoogle Scholar
  9. V.T. Oi, P.P. Jones, J.W. Goding, L.A. Herzenberg, and L.A. Herzenberg. Curr. Topics Microbiol. Immunol. 81:115 (1978).Google Scholar
  10. D.R. Parks, V.M. Bryan, V.T. Oi, and L.A. Herzenberg. Proc. Natl. Acad. Sci. 76:1962 (1979).PubMedCrossRefGoogle Scholar
  11. I. Scher, A.K. Berning, S. Kessler, and F.D. Finkelman. J. Immunol. 125:1686 (1980).PubMedGoogle Scholar
  12. E.S. Vitetta, U. Melcher, M. McWilliams, M.E. Lamm, J.M. Phillips-Quagliata, and J.W. Uhr. J. Exp. Med. 141:206 (1975).PubMedCrossRefGoogle Scholar

Copyright information

© Plenum Press, New York 1982

Authors and Affiliations

  • J. J. Haaijman
    • 1
  • W. J. A. Boersma
    • 1
  1. 1.Institute for Experimental Gerontology TNPRijswijkThe Netherlands

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