Separation and Characterization of Tonsillar Germinal Centre Lymphocytes Using Peanut Lectin
The lectin derived from the peanut plant (Arachis hypogaea) has been shown to bind to the surface of lymphocytes within germinal centres of mouse and human lymph nodes (1,2) but not to other lymphocytes in peripheral lymphoid tissue. Here we have studied the properties of PNL binding cells from human hyperplastic tonsils. Electron microscopy of the PNL binding cells revealed a series of lymphoblasts and putative plasma cells with morphology very similar to that which has already been described for tonsil germinal centre cells (4). Tonsillar mononuclear cells were separated on PNL coated plastic dishes, using galactose to release the adherent cells (3). In this way three fractions were obtained, those depleted in PNL+ cells, those enriched for PNL+ cells and the unfractionated control cells. These fractions were examined for the presence of cell surface antigens characteristic of T and B lymphocytes, for their mitogenic response to phytohaeraagglutinin (PHA) and pokeweed mitogen (PWM) and for their ability to generate sheep erythrocyte (SRBC) specific plaques after culture with PWM.
KeywordsAdherent Cell Arachis Hypogaea Peanut Plant Pokeweed Mitogen Peripheral Lymphoid Tissue
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- 2.M.L. Rose, J.A. Habeshaw, R. Kennedy, E. Wiltshaw, J. Sloane, and A.J.S. Davies. Brit. J. Cancer (1981) in press.Google Scholar