Immobilized Artificial Membrane Chromatography
Immobilized artificial membranes (IAM) are solid-phase-membrane-mimetics.1 Synthesis of IAM particles entails bonding cell membrane lipid molecules to solid surfaces at high molecular surface densities.1–4 Thus IAM surfaces are intended to mimic the lipid environment of cell membranes and consequently the initial applications of IAM particles relate to endogenous solute—membrane interactions. Non-chromatographic applications of IAM include reconstitution of phospholipase D (unpublished observation), and the correlation of drug-binding to IAM with drug-transport through human skin.5 Chromatographic applications include the purification of cytochrome P-450 (submitted for publication and also refs.6,7), and the purification of other membrane proteins.8 Although both non-chromatographic and chromatographic applications are evolving, the most useful application (s) of IAM will be for the purification of membrane proteins.
KeywordsMembrane Protein Myelin Basic Protein Critical Micelle Concen Coomassie Staining Detergent Concentration
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