Second Messengers in Newt Limb Regeneration: cAMP and cGMP Levels and Distribution
cAMP and cGMP measured by RIA and observed by immunohis-tochemistry in unamputated limbs and in regenerating newt limbs at early- and medium-bud stages showed cAMP and cGMP cytoplasmic accumulations in blastemata and in stumps of the regenerating limbs, with increases up to 1900% over unamputated controls. In blastemata the cAMP increase was greater than that of cGMP while in stumps of the regenerating limbs the increase of cGMP was of a much greater magnitude than that of cAMP. These increases were associated with cell cycling (mainly in blastemata), cell dedifferentiation and cell movements (mainly in stumps).
KeywordsCyclic Nucleotide Tritiated Thymidine cGMP Level Distal Stump Cell Dedifferentiation
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