Survey of Research Work on the Regeneration of Triturus Alpestris Forelimbs Treated with Vitamin A Palmitate

  • Stauros Koussoulakos
Part of the NATO ASI Series book series (NSSA, volume 172)


These experiments were conducted to widen our understanding of the action of retinoids on urodele limb regeneration. Vitamin A palmitate was orally administered to terrestrial stages of adult Triturus alpestris, and the results were evaluated at both the morphological and cellular levels. It was observed, that the drug accelerated the speed of regeneration at the zeugopodial level, whereas, at the stylopod, growth rates lagged behind that of control animals. Proximalization was induced in 3 0% of the treated animals, but with a higher frequency on the right limb. In accord with the increased speeds of forearm regeneration, higher mitotic indices were scored in both mesenchymal and epidermal cells. On the contrary, treated animals displayed lower labelling indices than control ones. Microdensitometric scanning of the nuclear DNA in mesenchymal cells revealed a diminution in the duration of the S-phase, whereas, the time needed for cells to pass through G2-phase was prolonged. The first days of vitamin A treatment were characterized by the appearance of some polyploid mesenchymal cells.


Labelling Index Limb Regeneration Blastema Cell Axolotl Limb Amphibian Limb 
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Copyright information

© Plenum Press, New York 1989

Authors and Affiliations

  • Stauros Koussoulakos
    • 1
  1. 1.Zoological LaboratoryUniversity of AthensAthensGreece

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