An Examination of Heat Shock and Trauma-Induced Proteins in the Regenerating Forelimb of the Newt, Notophthalmus Viridescens
Heat shock protein (hsp) synthesis and accumulation was studied in the limb tissues of the newt, Notophthalmus viridescens, in response to the stresses of hyperthermia and amputation. We also examined the developmental regulation and heat inducible expression of these proteins during subsequent stages of regeneration. Both heat shock and amputation cause a decrease in total protein synthesis and the selective synthesis of 70 kD proteins. Two-dimensional gel electrophoretic analysis reveals differences in these two sets of 70 kD proteins, the amputation induced form (amp 70) being more acidic than hsp 70. These stresses, in addition, have quite different effects on the accumulation of hsp 70 in limb stump tissues. Heat shock only slightly elevates the levels of what appears to be the constitutive form of hsp 70 (hsc 70) in the limb, whereas amputation leads to a large decrease in the levels of this protein within one hour. The titre of the putative constitutive hsc 70 rises gradually after the wound healing stage and eventually increases to a level greater than controls during the late bud stage of regeneration. The results are discussed in light of a possible role for hsps and trauma induced proteins in the epimorphic regeneration of the amphibian limb.
KeywordsHeat Shock Hsp70 Synthesis Human Hsp70 Blastema Cell Total Protein Synthesis
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- Heikkila, J.J., Browder, L.W., Gedamu, L., Nickells, R.W., and Schultz, G.A., 1986, Heat-schock gene expression in animal embryonic systems, Cab. J. Genet. Cytol., 28:1093–1105.Google Scholar